The human colon cancer cell lines HCA-7 colony 29 (high COX-2 expression, European Collection of Cell Cultures, Salisbury, UK) and HT-29 (low COX-2 expression, ATCC) were grown in Dulbecco's Modified Eagle's Medium and McCoy’s 5A Medium (with 10% fetal bovine serum, Thermo Fisher Scientific, UT, USA), respectively. Cells were cultured in an incubator containing a 95% humidified atmosphere with 5% CO2 at 37 °C.
DGLA was purchased from Nu-Chek-Prep (MN, USA); 8-HOA and 5-FU were purchased from Sigma-Aldrich (MO, USA); regorafenib was obtained from Adooq Biosciences (CA, USA); irinotecan, PGE1, DGLA-d6 and PGE1-d4 were purchased from Cayman Chemicals (MI, USA).
DGLA was purchased from Nu-Chek-Prep (MN, USA); 8-HOA and 5-FU were purchased from Sigma-Aldrich (MO, USA); regorafenib was obtained from Adooq Biosciences (CA, USA); irinotecan, PGE1, DGLA-d6 and PGE1-d4 were purchased from Cayman Chemicals (MI, USA).