Cytokine antibody arrays

Manufactured by R&D Systems

Sourced in United States

Cytokine antibody arrays are laboratory equipment used to simultaneously detect and measure multiple cytokines in a single biological sample. These arrays contain immobilized antibodies specific to different cytokines, allowing for the analysis of cytokine expression profiles in a high-throughput manner.

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Lab products found in correlation

Human tnf alpha duoset, by R&D Systems (1 mentions) Human il 10, by Thermo Fisher Scientific (1 mentions) Enhanced chemi luminescence (ecl), by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Proteome Profiler Antibody Arrays (Human XL Cytokine Array Kit, Cytokine arrays

3 protocols using cytokine antibody arrays

Immunoblotting and Cytokine Analysis

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Immunoblotting was performed as described (19 (link)). Cytokines, ELISA kits and cytokine antibody arrays were purchased from R&D Systems.

Zhu Z., Aref A.R., Cohoon T.J., Barbie T.U., Imamura Y., Yang S., Moody S.E., Shen R.R., Schinzel A.C., Thai T.C., Reibel J.B., Tamayo P., Godfrey J.T., Qian Z.R., Page A.N., Maciag K., Chan E.M., Silkworth W., Labowsky M.T., Rozhansky L., Mesirov J.P., Gillanders W.E., Ogino S., Hacohen N., Gaudet S., Eck M.J., Engelman J.A., Corcoran R.B., Wong K.K., Hahn W.C, & Barbie D.A. (2014). Inhibition of KRAS-driven tumorigenicity by interruption of an autocrine cytokine circuit. Cancer discovery, 4(4), 452-465.

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Cytokine and Chemokine Profiling of DC and CIK Cells

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Cytokine and chemokine expression was analyzed two days after adenoviral transduction in the supernatant of DC as well as in the supernatant of CIK cells after coculturing for four days with transduced DC using commercial ELISA kits: human TNFalpha DuoSet (R&D Systems, Wiesbaden, Germany; 88-7126-22, DY210-05, DY008), human Interferon-gamma, eBiosciences (San Diego, USA, 14-7318-67) and human IL-10 from eBiosciences (San Diego, USA, 14-7108-67),according to the manufacturer’s instructions. For multianalyte cytokine detection, using the cytokine multiplex assay kit, the Cytokine Antibody Arrays (R&D Systems, Minneapolis, USA) were used. The sample mixtures were incubated with the cytokine array membrane and bound by capture antibody on the membrane, if present. After a wash step to remove unbound material, Streptavidin–HRP and chemiluminescent detection reagents were added. Light was produced at each spot in proportion to the amount of cytokine bound. The cytokine array was analyzed by chemiluminescence. Amounts of cytokine were semiquantified and compared using a densitometry software.

Sadeghlar F., Vogt A., Mohr R.U., Mahn R., van Beekum K., Kornek M., Weismüller T.J., Branchi V., Matthaei H., Toma M., Schmidt-Wolf I.G., Kalff J.C., Strassburg C.P, & González-Carmona M.A. (2020). Induction of cytotoxic effector cells towards cholangiocellular, pancreatic, and colorectal tumor cells by activation of the immune checkpoint CD40/CD40L on dendritic cells. Cancer Immunology, Immunotherapy, 70(5), 1451-1464.

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Cytokine Profiling Using Antibody Array

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To evaluate cytokine pro les, cytokine antibody arrays (R&D) were used. TANCS, NeuCS, MDA231CS and SFM were collected and diluted with array buffer. After blocking with array buffer for 1 hour, the array membrane was incubated with 1.5 ml dilution overnight at 4°C. After incubation with HRP-conjugated detection antibodies for 1 hour, outcomes were visualized by ECL (Thermo Fisher). The signals, as relative units of spot color density, were evaluated by ImageJ software.

Sheng Y., Peng W., Huang Y., Cheng L., Meng Y., Yin Y., Yang J., Xu J., Han X., Kzhyshkowska J., & Wu Q. (2022). Tumor-activated Neutrophils Promote Metastasis in Breast Cancer via the G-CSF-RLN2-MMP-9 Axis.

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