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3 3 diaminobenzide

Manufactured by Merck Group

3,3′diaminobenzide is a chemical compound used in various laboratory applications. It serves as a precursor for the synthesis of other organic compounds. The core function of this product is to provide a reliable and consistent starting material for further chemical reactions and analyses. No additional information or interpretation is provided.

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2 protocols using 3 3 diaminobenzide

1

Immunophenotyping of T Cells and Bone Marrow Cells

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OCT embedded tissues were cut at ~8-10 μm, fixed with paraformaldehyde/zinc; blocked with Aurion® blocking solution containing normal donkey serum; and incubated either with goat anti-rabbit RTLA (Rabbit TLymphocyte Antigen, Cedarlane Labs, Burlington, NC) as a marker for T cells or with mouse anti-rabbit CD18 (Antigenix America, Huntington Station, NY) as a marker for bone marrow-derived cells. Biotin-SP-conjugated donkey-anti-goat IgG, donkey anti-mouse IgG, and peroxidase-conjugated streptavidin were from Jackson ImmunoResearch Laboratories (West Grove, PA). 3,3′diaminobenzide was from Sigma-Aldrich (St. Louis, MO). Sections were counterstained with Hematoxylin QS® (Vector Laboratories, Burlingame, CA). The percentages of total cells immunopositive for RTLA and CD18 were determined with MetaMorph® Microscopy Automation and Image Analysis Software.
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2

Immunophenotyping of T Cells and Bone Marrow Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
OCT embedded tissues were cut at ~8-10 μm, fixed with paraformaldehyde/zinc; blocked with Aurion® blocking solution containing normal donkey serum; and incubated either with goat anti-rabbit RTLA (Rabbit TLymphocyte Antigen, Cedarlane Labs, Burlington, NC) as a marker for T cells or with mouse anti-rabbit CD18 (Antigenix America, Huntington Station, NY) as a marker for bone marrow-derived cells. Biotin-SP-conjugated donkey-anti-goat IgG, donkey anti-mouse IgG, and peroxidase-conjugated streptavidin were from Jackson ImmunoResearch Laboratories (West Grove, PA). 3,3′diaminobenzide was from Sigma-Aldrich (St. Louis, MO). Sections were counterstained with Hematoxylin QS® (Vector Laboratories, Burlingame, CA). The percentages of total cells immunopositive for RTLA and CD18 were determined with MetaMorph® Microscopy Automation and Image Analysis Software.
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