Pc 12 adh atcc crl 1721.1

Cell lines were cultured and treated with drugs as described in Supplementary data. PC-12 Adh (ATCC CRL-1721.1) cells were obtained from the American Type Culture Collection; for cilia assembly experiments, cells were plated and grown in complete media for 24 h prior to serum starvation for a further 24 h or otherwise specified. For cilia disassembly experiments serum-containing media was reintroduced for 24 h after starvation or as otherwise specified.

Poly(ε-caprolactone) (M_w 80 kDa), glutaraldehyde, sorbitan monooleate (Span 80), hexamethyldisilazane (HMDS), sodium dodecyl sulfate (SDS), Dulbecco’s modified eagle’s medium (DMEM/F12) were purchased from Sigma, Singapore. Chloroform (CHCl₃) was purchased from Fisher Scientific Company, Loughborough, UK. Micro BCA Protein Assay Kit was purchased from Thermo Scientific (Rockford, lL, USA). Sodium hydroxide (NaOH) was bought from Merck (Singapore). Nerve growth factor (NGF 7S) and ChemiKine Nerve Growth Factor Sandwich ELISA kit were purchased from Millipore, Singapore. Rat pheochromocytoma (PC12) cells in the adherent type [PC12 Adh (ATCC^® CRL1721.1™)] were obtained from ATCC, Manassas, VA, USA, while fetal bovine serum (FBS), horse serum (HS), and trypsin/EDTA were purchased from Invitrogen/Gibco, Grand Island, NY, USA. [3-(4,5-Dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt; MTS] was purchased from Promega, Madison, WI, USA. Alexa Fluor 594 Goat anti-Mouse IgG antibody was obtained from Invitrogen, Eugene, OR, USA.