DSPC (Avanti Corporation, USA), DSPE-PEG2000 (Avanti Corporation, USA), DSPE-PEG2000-maleimide (Avanti Company, USA), DC-cholesterol∙HCl (Avanti Company, USA), DPPE (Avanti Company, USA), glycerol (Sigma, USA), sodium hyaluronate (HA, 200–400 kDa, Xi’an Ruixi), aptamer PM1 base sequence SH-CCCCTGCAGGTGATTTTGCTCAAGTCGTTCCCGTCCTCTCCTCTGCGCCCCGTCTCGGCCAGTATCGCTAATCAGGCGGAT-HS, (Shanghai Shenggong, China), a sequence with FITC-labelled or unlabelled at the 5ʹ end, ANM33 and ANM33 double-modified with cy3 and amino groups (Guangzhou Ruibo, China), DiI fluorescent dye (Yeasen, USA), RAW264.7-T1 macrophages (Procell Life Science & Technology, China), oxidized low-density lipoprotein (ox-LDL; Yiyuan Biotechnology, China), Oil red O staining kit (Sigma, China), high-glucose DMEM (ScienCell, China), and double antibodies (ScienCell, USA) were used. A fluorescence microscope (LEICA CTR6000, Germany), laser confocal microscope (Leica SP8, Germany), flow cytometer (Beckman, USA), and laser nanoparticle size and zeta potential analyser (Malvern, UK) were also used.
High glucose dmem
Manufactured by ScienCell
Sourced in China, United States
High-glucose DMEM is a cell culture medium formulated with a high concentration of glucose (4.5 g/L) to support the growth and maintenance of a variety of cell types. It provides essential nutrients, vitamins, and amino acids necessary for cell proliferation and metabolism.
Automatically generated - may contain errors
Lab products found in correlation
Double antibodies, by ScienCell (2 mentions)
Fetal bovine serum (fbs), by ScienCell (2 mentions)
Sodium hyaluronate ha, by Merck Group (1 mentions)
Oil red o staining kit, by Merck Group (1 mentions)
Dii fluorescent dye, by Yeasen (1 mentions)
Ctr6000, by Leica (1 mentions)
Glycerol, by Merck Group (1 mentions)
Double antibody, by Thermo Fisher Scientific (1 mentions)
Endothelial cell growth factor, by ScienCell (1 mentions)
Tnf α, by Bioss Antibodies (1 mentions)
Transwell chamber, by Corning (1 mentions)
2 protocols using high glucose dmem
1
Aptamer-Mediated Targeted Delivery of Lipid Nanoparticles
2
Endothelial-Macrophage Interplay in Ox-LDL Exposure
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Human umbilical vein endothelial HUVEC-T1 cells (Procell Life Science & Technology, China), RAW264.7-T1 Mφ (Procell Life Science & Technology, China), oxidized low-density lipoprotein (ox-LDL; Yiyuan Biotechnology, China), tumor necrosis factor-α (TNF-α; Bioss, China), and Transwell chambers (Corning, USA) were used. FITC-labelled anti-human CD44 flow cytometry antibody (Proteintech, USA), high-glucose DMEM (ScienCell, China), CCK-8 kit (Tongren Chemical, Japan), fetal bovine serum (FBS; ScienCell, USA, Israel), and double antibody (Gibco, USA) were purchased for this study. Endothelial cell basal medium (ECM; ScienCell, USA), FBS (ScienCell, USA), endothelial cell growth factor (ScienCell, USA), double antibodies (ScienCell, USA), and Fluorescence microscope (LEICA CTR6000, Germany); laser confocal microscope (Leica SP8, Germany); flow cytometer (Beckman, USA); laser nanoparticle size potential analyzer (Malvern, UK) were used.
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