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4 protocols using methyl orange

1

Antimicrobial Efficacy of Silver Nanoparticles

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Klebsiella pneumoniae (PME2), K. pneumoniae (MBC34), Micrococcus luteus (MBC23), Micrococcus luteus (MBC57), Enterobacter aerogenes (MBX6), Bacillus subtilis (MBC3), Bacillus cereus (MBC4), Bacillus megaterium (MBP11), Enterococcus faecalis (MBP13), and Serratia marcescens (MBC27) were obtained as gift samples from the Gujarat Biotech Research Centre (Gandhinagar, Gujarat, India). The materials used in the study included silver nitrate (SRL, Gujarat, India), nutrient agar media, nutrient broth and antibiotic assay media (Himedia, Mumbai, India); ethanol (SLC Chemicals, Delhi, India), Whatman filter paper no. 42. (Axiva, Mumbai, India); and methyl orange (MO) (Loba, Chemie, Gujarat, India) and double distilled water (ddw). All the chemicals were analytical grade except silver nitrate and methyl orange dye (LR grade).
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2

Synthesis of Multifunctional Nanocomposites

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The chemicals
used in the present work, such as lanthanum nitrate hexahydrate (99.9%)
[La(NO3)3·6H2O], iron nitrate
nonahydrate (>98%) [Fe(NO3)3·9H2O], chromium nitrate nonahydrate (98.5%) [Cr(NO3)3·9H2O], glycine (99%), poly(vinyl alcohol)
(PVA) (98–99%), urea (99%), rhodamine B (RhB), and hydrogen
peroxide (27% w/w), were purchased from Alfa Aesar. Methylene blue
(MB) and methyl orange (MO) were from Loba Chemie. All the chemicals
were reagents of analytical quality and were used directly without
further purification.
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3

Synthesis of Multicomponent Metal Oxide Nanoparticles

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Zinc nitrate hexahydrate
(Alfa Aesar, 99%), bismuth nitrate pentahydrate (Sigma-Aldrich, 98%),
ferric nitrate nonahydrate (Sigma-Aldrich, ≥98%), glycine (Sigma-Aldrich,
99%), sucrose (Sigma-Aldrich, 99.5%), 5,5-dimethyl-1-pyrroline N-oxide
(Sigma-Aldrich, ≥97%, DMPO), 2,2,6,6-tetramethylpiperidine
(Sigma-Aldrich, 98%, TEMP), and methyl orange (Loba Chemie) were used
as received. The water used for synthesis and other experiments was
deionized (DI).
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4

Waste Fly Ash Adsorption Study

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The Methylene Blue (C 16 H 18 CIN 3 S, MW: 319.85 g/mol, Dye content: 82%, λmax = 660nm), Methyl orange (C 14 H 14 N 3 NaO 3 S, MW: 327.34g/mol, λ max = 460nm) and Rhodamine-6G (C 28 H 30 N 2 O 3 HCl, MW: 479.02 g/mol, λ max = 523 nm) were provided by LOBA Chemie and BDH Chemicals respectively and were used without prior puri cation. Analytical grades of Nitric and Phosphoric acids were obtained from Panreac Company, Spain. Ammonia solution (32% w/w) of density 0.9g/cm 3 was delivered by Scharlau Company, Spain. The power plant waste y ash (WFA) used in the experiments was obtained from the Rabigh power plant located in Jeddah, Saudi Arabia.
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