Anti cd8 fitc

The PBMCs were stained with CMV, GPC3 or HIV Dextramer-RPE (Immudex, Copenhagen, Denmark) for 10 min at room temperature and with anti-CD8-FITC (ProImmune) or anti-CD8-APC (BioLegend, San Diego, CA), anti-CD45RA-FITC (BD Biosciences, San Jose, CA, USA), and anti-CCR7-PerCP/Cy5.5 (BioLegend) for 20 min at 4°C. To detect γδ T cells, PBMCs were stained with anti-TCR-Vγ9-FITC (Beckman Coulter, Erembodegem, Belgium) and anti-CD3-PC5 (BioLegend) for 20 min at 4°C. γδ T cells, with or without zoledronate activation, and TNF-DCs were stained with anti-HLA-class I-FITC, anti-CD80-FITC, anti-CD83-FITC and anti-CD86-PE (BD Biosciences) antibodies for 20 min at 4°C. Flow cytometry analysis was carried out using FACSCanto II (BD Biosciences).