CE‐TOFMS was performed using an Agilent CE Capillary Electrophoresis System with an Agilent 6210 Time of Flight mass spectrometer, Agilent 1100 isocratic HPLC pump, Agilent G1603A CE‐MS adapter kit, and Agilent G1607A CE‐ESI‐MS sprayer kit (Agilent Technologies, Waldbronn, Germany), as described in the previous papers.17 , 18 , 19 , 20 The systems were controlled by the Agilent G2201AA ChemStation software version B.03.01 for CE (Agilent Technologies). The metabolites were analyzed by using a fused silica capillary with the electrophoresis buffer (Human Metabolome Technologies) as the electrolyte. The sample was injected at a pressure of 50 mbar for 10 s in cation analysis and 25 s in anion analysis. The spectrometer was scanned from m/z 50 to 1000.
Electrophoresis buffer
Manufactured by Human Metabolome Technologies
Electrophoresis buffer is a solution used in electrophoretic separation techniques. Its core function is to provide the necessary ionic environment and pH conditions to facilitate the migration and separation of charged biomolecules, such as proteins, nucleic acids, or ions, during the electrophoresis process.
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Lab products found in correlation
Agilent g1607a ce esi ms sprayer kit, by Agilent Technologies (2 mentions)
Agilent g2201aa chemstation software, by Agilent Technologies (2 mentions)
Agilent 1100 isocratic hplc pump, by Agilent Technologies (2 mentions)
Agilent g1603a ce ms adapter kit, by Agilent Technologies (2 mentions)
Agilent ce capillary electrophoresis system, by Agilent Technologies (1 mentions)
Agilent 6210 time of flight mass spectrometer, by Agilent Technologies (1 mentions)
2 protocols using electrophoresis buffer
1
CE-TOFMS Protocol for Metabolite Analysis
2
Metabolite Analysis by CE-MS
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Time of Flight mass spectrometer, Agilent 1100 isocratic HPLC pump, Agilent G1603A CE-MS adapter kit, and Agilent G1607A CE-ESI-MS sprayer kit (Agilent Technologies, Waldbronn, Germany), as described in previous papers (31) (32) (33) . The systems were controlled by the Agilent G2201AA ChemStation software version B.03.01 for CE (Agilent Technologies). The metabolites were analyzed by using a fused silica capillary with the electrophoresis buffer (Human Metabolome Technologies) as the electrolyte. The sample was injected at a pressure of 37.5mmHg (50mbar) for 10 seconds in cation analysis and 25 seconds in anion analysis. The scanning of spectrometer was performed from m/z 50 to 1,000.
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