Anti icos l

Manufactured by BioLegend

anti-ICOS-L is a laboratory research reagent that binds to the ICOS-L (Inducible T-cell COStimulator Ligand) protein. ICOS-L is a member of the B7 family of costimulatory molecules and plays a role in the regulation of T-cell activation and function. The anti-ICOS-L reagent can be used in various in vitro and in vivo research applications to study the biological functions of the ICOS-L protein.

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Lab products found in correlation

Anti cd3 bv510, by BioLegend (1 mentions) Alexa fluor 700, by BioLegend (1 mentions) Anti pd l2, by BioLegend (1 mentions) Anti foxp3 pe, by Thermo Fisher Scientific (1 mentions) Percpcy5.5 anti cd66b, by BioLegend (1 mentions) Alexa fluor 488 anti cd11b, by BioLegend (1 mentions) Anti cd19 bv605, by BioLegend (1 mentions) Anti cx3cr1 pe, by BioLegend (1 mentions) Anti pd l1, by BioLegend (1 mentions) Isotype control mab, by BioLegend (1 mentions)

2 protocols using anti icos l

Multiparameter Flow Cytometry Profiling of Immune Cells

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Antibodies used for flow cytometry were Alexa Fluor488-anti-CD11b, PerCP-eF710/PE/or PE-Dazzel594-anti-HLA DR (clone L243), eF450-anti-HLA DR (clone LN3) PE/Cy7-anti-CD14, APC-anti-CD33, BV510-anti-CD3, BV605-anti-CD19, PerCP-Cy5.5-anti-CD66b, Alexa Fluor700 or PerCP-Cy5.5-anti-IFNγ, APC/Cy7-CD38, PerCP-Cy5.5-anti-CD69, PE-anti-CX3CR1, -anti-B7-H4, -anti-PD-L1, -anti-B7-H3, -anti-PD-L2, -anti-ICOS-L (all from Biolegend, San Diego, CA); FITC- or APCeF780-anti-CD4, Alexa Fluor700- or eF450-anti-CD25, PE-anti-FoxP3 and eF450-anti-IL10 (all from eBioscience); PE-anti- phospho-Zap70^(pY292), PE-anti-phospho-Akt^(pS473) from BD Biosciences. For neutralization studies, mAb to IL-10, IL-6 or rat IgG isotype (10 μg/mL, Biolegend) and polyclonal goat anti-IL-27 or normal goat IgG control (10 μg/mL R&D Systems) control were used.

Garg A., Trout R, & Spector S.A. (2017). Human Immunodeficiency Virus Type-1 Myeloid Derived Suppressor Cells Inhibit Cytomegalovirus Inflammation through Interleukin-27 and B7-H4. Scientific Reports, 7, 44485.

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Inhibition of ICOS/ICOS-L pathway in mice

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For inhibition of the ICOS/ICOS-L pathway, mice were injected on d 0 with 250 μg of anti-ICOS-L or the appropriate isotype control mAb (BioLegend, San Diego, CA). Mice were then injected in the footpad with papain, and cytokine production in the PLN was assessed after 5 d.

Dwyer D.F., Woodruff M.C., Carroll M.C., Austen K.F, & Gurish M.F. (2014). B Cells Regulate CD4+ T cell Responses to Papain Following BCR-Independent Papain Uptake. Journal of immunology (Baltimore, Md. : 1950), 193(2), 529-539.

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