Human cytokine arrays spotted on nitrocellulose membranes

Manufactured by RayBiotech

Human cytokine arrays spotted on nitrocellulose membranes. The core function of these arrays is to enable the simultaneous detection and quantification of multiple human cytokines in a sample.

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2 protocols using human cytokine arrays spotted on nitrocellulose membranes

Cytokine profiling of cancer cells

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Human cytokine arrays spotted on nitrocellulose membranes were obtained from Raybiotech (Norcross, GA). Conditioned medium was prepared from SKLU-vector, SKLU-DACH1, A549-vector, and A549-DACH1 cells by culturing cells in serum free 1640 for 24–48 h. Cytokine array analysis was performed as described previously [21 (link)].

Liu Q., Li A., Yu S., Qin S., Han N., Pestell R.G., Han X, & Wu K. (2018). DACH1 antagonizes CXCL8 to repress tumorigenesis of lung adenocarcinoma and improve prognosis. Journal of Hematology & Oncology, 11, 53.

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Cytokine Profiling of DACH1 Expressing Cells

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Human cytokine arrays spotted on nitrocellulose membranes were obtained from Raybiotech (Norcross, GA) (45 (link)). Conditioned medium was prepared from cells transduced with an expression vector encoding DACH1 or DACH1 ΔDS by culturing cells in serum free DMEM for 24–48 hrs. The conditioned medium from Dach1^+/+ and Dach^−/− PEC was prepared from cells grown in serum free media for 24 hrs.

Chen K., Wu K., Jiao X., Wang L., Ju X., Wang M., Di Sante G., Xu S., Wang Q., Li K., Sun X., Xu C., Li Z., Casimiro M.C., Ertel A., Addya S., McCue P., Lisanti M.P., Wang C., Davis R.J., Mardon G, & Pestell R.G. (2015). The Endogenous Cell-Fate Factor Dachshund restrains Prostate Epithelial Cell Migration via Repression of Cytokine Secretion via a CXCL Signaling Module. Cancer research, 75(10), 1992-2004.

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