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Trypan blue exclusion assay

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Trypan blue exclusion assay is a technique used to determine the viability of cells. It involves the use of a dye, trypan blue, which selectively stains dead or damaged cells. Live cells with intact cell membranes are able to exclude the dye, while dead or damaged cells take up the dye and appear blue under a microscope. This assay provides a simple and rapid method for assessing cell viability.

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Lab products found in correlation

Celltiter 96 aqueous one solution cell proliferation assay, by Promega (1 mentions) Fitc annexin 5 apoptosis detection kit 1, by BD (1 mentions) Cell titer 96 aqueous one solution cell assay, by Promega (1 mentions)

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Trypan blue (41 citations)

2 protocols using trypan blue exclusion assay

1

Cell Viability and Apoptosis Assay

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Both MM and MM-3D cells on 48-well plates after drug treatment were recovered by cell recovery solution and resuspended in fresh growth medium. 100 µL resuspended cells were transferred to a 96-well plate. Cell viability was measured by CellTiter 96® AQueous One Solution Cell Proliferation Assay (i.e., MTS assay, Promega, Madison, WI, USA) or trypan blue exclusion assay (Amresco, Solon, OH, USA). The apoptosis was measured by following the instructions of FITC Annexin V Apoptosis Detection Kit I (BD Biosciences, San Jose, CA, USA).
Huang Y.H., Molavi O., Alshareef A., Haque M., Wang Q., Chu M.P., Venner C.P., Sandhu I., Peters A.C., Lavasanifar A, & Lai R. (2018). Constitutive Activation of STAT3 in Myeloma Cells Cultured in a Three-Dimensional, Reconstructed Bone Marrow Model. Cancers, 10(6), 206.
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2

Cell Viability Assays for Drug Treatments

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The CellTiter 96® AQueous One Solution Cell Assay (i.e MTS assay) (Promega, Madison, WI, USA) was used to measure cell viability following drug treatments. The trypan blue exclusion assay (Amresco, Solon, OH, USA) was used to measure cell viability for time-dependent experiments.
Haque M., Li J., Huang Y.H., Almowaled M., Barger C.J., Karpf A.R., Wang P., Chen W., Turner S.D, & Lai R. (2019). NPM-ALK Is a Key Regulator of the Oncoprotein FOXM1 in ALK-Positive Anaplastic Large Cell Lymphoma. Cancers, 11(8), 1119.
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