Rabbit polyclonal von Willebrand factor antibody (Abcam, Cambridge, England), anti-CD31 (PECAM) antibody (Abcam), anti-platelet-derived growth factor (PDGF)-beta antibody (Abcam), and anti-alpha smooth muscle actin (aSMA) antibody (Abcam) were purchased, and Alexa Fluor 488-or Alexa Fluor 568-labeled goat anti-rabbit antibodies from Invitrogen were used. The 5-μm sections were fixed in 4% PFA, then incubated with primary antibodies for immunohistochemistry and appropriate secondary antibodies. An Axioskop 2 microscope (Carl Zeiss Microimaging, Welwyn Garden City, UK) equipped with an AxioCam (Carl Zeiss Microimaging) was used to observe tissues and take photographs. Fluorescence intensity was measured using Photoshop version 7.0 software (Adobe Systems Incorporated, San Jose, CA). The intensities of control samples were designated as 1. The results per view are expressed as mean ± standard deviation (SD).
Alexa fluor 488 or alexa fluor 568 labeled goat anti rabbit antibodies
Manufactured by Thermo Fisher Scientific
Sourced in United Kingdom
Alexa Fluor 488-or Alexa Fluor 568-labeled goat anti-rabbit antibodies are secondary antibodies that are conjugated with Alexa Fluor fluorescent dyes. These antibodies can be used to detect and visualize rabbit primary antibodies in various immunoassays and imaging applications.
Automatically generated - may contain errors
2 protocols using alexa fluor 488 or alexa fluor 568 labeled goat anti rabbit antibodies
1
Immunofluorescence Analysis of Vascular Markers
2
Quantification of Apoptosis Markers
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Rabbit polyclonal antibodies against cleaved caspase-3 (Trevigen, Gaithersburg, MD, USA), Akt, phosphorylated Akt (Akt-Thr308, Akt-Ser473), and paxillin (Abcam) were purchased. Alexa Fluor 488- or Alexa Fluor 568-labeled goat antirabbit antibodies were purchased from Invitrogen. For immunohistochemistry, 5 µm sections were fixed with 4% paraformaldehyde (PFA), incubated with primary antibodies, and then incubated with the appropriate secondary antibodies. Hoechst 33342 and Alexa Fluor 568-labeled phalloidin were used to visualize nuclei and polymerized actin, respectively. An Axioscope 2 (Carl Zeiss Microimaging Inc., Welwyn Garden City, UK) equipped with an AxioCam (Carl Zeiss) was used to observe tissues and take photographs.
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