2 phenylethanol

Yeast strains were pre-cultured in a liquid broth [YPD; 1% yeast extract, 2% peptone, and 2% dextrose]. Filamentous growth was induced on 4X synthetic low-ammonium dextrose agar [SLAD agar; 0.68% yeast nitrogen base w/o amino acids or ammonium sulfate, 2% dextrose (Biowest, Nuaillé, FR), 50 µM ammonium sulfate, and 2% washed agar (WVR International, Radnor, PA, USA] and when applicable, supplemented with 2-phenylethanol (Alfa Aesar, A15241) to a concentration of 100 µM or 200 µM just prior to plate pouring. Unless otherwise indicated, all materials were obtained from Sigma Aldrich, St. Louis, MO, USA.

Ultrapure 18.3 MΩ⋅cm water used for these experiments was produced from a Zeneer Power III TOC system (Human Corp., Seoul, Korea). All the following reagents were purchased from Sigma Aldrich, unless stated otherwise, and were standard grade: butyl acetate, hexanal (Alfa Aesar, Haverhill, MA, USA), butanol, 3-methyl-1-butanol (Alfa Aesar), (E)-hex-2-enal (Alfa Aesar), hexanol, (Z)-hex-3-en-1-ol (Alfa Aesar), nonanal, 2-ethyl-1-hexanol, acetophenone, α-terpineol, benzyl alcohol, 2-phenylethanol, ethyl tetradecanoate, ethyl dodecanoate, methyl 2-hydroxybenzoate (Alfa Aesar), citronellol, ethyl decanoate, benzaldehyde (Alfa Aesar), ethyl octanoate, ethyl heptanoate, isoamyl acetate, limonene, 2-methyl-1-butanol, ethyl hexanoate, ethyl acetate, ethyl butanoate, Saccharomyces cerevisiae (Franke, Italy), (NH₄)₂HPO₄ (Franke, Italy), K₂S₂O₅ (Franke, Italy), tartaric acid, polyvinylpolypyrrolidone (Alfa Aesar), sodium azide (Alfa Aesar), NaOH, Na₂PO₄(H₂O)₂, citric acid, HCl, 3-octanol, MeOH, CH₂Cl₂, β-glucosidase enzyme (Ecozim AROM, CRC Biotek, Roma, Italy), hydrocarbon mixture from C8–C23 (Sigma-Aldrich, St. Louis, MO, USA).