Plasma level IL-21 was measured in patients and controls using human IL-21 Duo Set ELISA kit (R&D Systems, Inc., USA) according to the manufacturer’s instructions. All plasma samples were measured in duplicate, and the average absorbance value was recorded for a study subject. Furthermore, various autoantibodies such as Anti-cyclic citrullinated peptide (Anti-CCP: Euroimmune, Germany), anti rheumatoid factors (IgG and IgM: Abnova, Germany) were quantified by ELISA according to the instructions of the manufacturer’s.
Human il 21 duo set elisa kit
Manufactured by R&D Systems
Sourced in United States
The Human IL-21 Duo Set ELISA kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the measurement of human interleukin-21 (IL-21) levels in cell culture supernatants, serum, and plasma.
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Lab products found in correlation
3 protocols using human il 21 duo set elisa kit
1
Plasma IL-21 and Autoantibody Profiling
2
Quantifying Plasma IL-21 Concentrations
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Plasma samples for the 11 Protocol C volunteers were thawed and tested in an IL-21 commercial ELISA kit. The Human IL-21 DuoSet ELISA kit from R&D Systems (Minneapolis, Minnesota, USA) was used to quantify circulating human IL-21 plasma concentrations using the manufacturers protocol. A standard curve was used to interpolate the concentrations of the plasma IL-21 with Graphpad Prism v8 software (GraphPad Software, San Diego, California, USA).
3
SNP Genotyping and IL-21 ELISA
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A total of four SNPs (rs907715, rs2221903, rs2055979 and rs6822844) were typed by TaqMan SNPs genotyping method. Predesigned SNP genotyping assays kit were procured from Thermo Fisher Scienti c Enzyme-Linked Immunosorbent Assay Plasma level IL-21 was measured in patients as well as controls using human IL-21 Duo Set ELISA kit (R&D Systems, Inc, USA) according to the manufacturer's instructions. All plasma samples were measured in duplicate, and the average absorbance value was recorded for a study subject.
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