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Ywhaz

Manufactured by Proteintech

YWHAZ is a protein that belongs to the 14-3-3 family. It is a highly conserved protein that plays a role in various cellular processes such as signal transduction, cell cycle regulation, and apoptosis. YWHAZ is commonly used as a housekeeping gene for normalization in gene expression analyses.

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2 protocols using ywhaz

1

Investigating Protein Interactions

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Co-immunoprecipitation (Co-IP) and immunoprecipitation (IP) were conducted using the IP/Co-IP kit (ABsin) according to the manufacturer's instructions. The primary anti-bodies used in this assay included β-catenin (ABclonal), YWHAZ (Proteintech), Ubiquitin (CST), β-tubulin (ABclonal).
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2

Protein Extraction and Western Blot

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Total proteins from cells were extracted using RIPA buffer supplemented with protease inhibitors and phosphatase inhibitors. The primary anti-bodies used in this assay included E-cadherin (ABclonal), vimentin (CST), N-cadherin (Proteintech), β-catenin (Proteintech), YWHAZ (ABclonal), GAPDH (Proteintech), Lamin B (Proteintech).
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