X-ray Diffraction (XRD) measurements were carried out using X’Pert Pro XRD equipped with x’Celerator solid-state detector. Fourier-Transform Infrared Spectroscopy (FT-IR) studies were performed using Perkin Elmer - Spectrum RX-FT-IR by casting sample on potassium bromide (KBr) pellets within a scan range of 4000–400 cm−1. KRÜSS-DSA25E was used to evaluate hydrophilic/phobic behaviour of nanoparticle membranes by measuring static contact angle of a water droplet at room temperature (37 °C). Transmission Electron Microscopy (TEM) was used to study the morphology by using TEM, Hitachi (H-7500) 120 kV. The samples were prepared using the drop casting method. Minimum Inhibitory Concentration (MIC) was evaluated by observing readings using ELISA plate reader.
Spectrum rx ft ir
Manufactured by PerkinElmer
The Spectrum RX-FT-IR is a Fourier Transform Infrared (FT-IR) spectrometer designed for analytical applications. It provides accurate and reliable infrared spectroscopy measurements. The core function of the Spectrum RX-FT-IR is to identify and quantify chemical compounds through the analysis of their infrared absorption spectra.
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2 protocols using spectrum rx ft ir
1
Nanoparticle Membrane Characterization Protocol
2
Characterization of Cysteamine-Capped Gold Nanoparticles
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UV-visible absorption measurements were recorded at room temperature in the wavelength region of 200 -800 nm using a Spectra Max i3X microplate reader. An X-ray powder diffractometer (XRD) with Cu-Kα radiation (Bruker AXS D8) was used to identify the crystalline phase of as-synthesized C-AuNPs.
The surface morphology of as-synthesized cysteamine-capped gold nanoparticles was examined using cold field-emission scanning electron microscopy (SEM) [Hitachi SU1080] coupled with EDX. The hydrodynamic size and surface charge of as-synthesized cysteamine-capped gold nanoparticles were measured by a dynamic light scattering (DLS) zetasizer [ZS90 series]. Centrifugation was carried out using an Eppendorf Centrifuge 5804 R. Attenuated total reflection (ATR)-FTIR spectra of cysteamine-capped gold nanoparticles and antibody labeled gold nanoparticles were recorded with a Perkin Elmer-Spectrum RX-FTIR mounted with a diamond crystal ATR unit. The colloidal solution was cast onto the diamond crystal, dried and subsequently characterized.
The surface morphology of as-synthesized cysteamine-capped gold nanoparticles was examined using cold field-emission scanning electron microscopy (SEM) [Hitachi SU1080] coupled with EDX. The hydrodynamic size and surface charge of as-synthesized cysteamine-capped gold nanoparticles were measured by a dynamic light scattering (DLS) zetasizer [ZS90 series]. Centrifugation was carried out using an Eppendorf Centrifuge 5804 R. Attenuated total reflection (ATR)-FTIR spectra of cysteamine-capped gold nanoparticles and antibody labeled gold nanoparticles were recorded with a Perkin Elmer-Spectrum RX-FTIR mounted with a diamond crystal ATR unit. The colloidal solution was cast onto the diamond crystal, dried and subsequently characterized.
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