The UPLC system consisted of the Acquity UPLC binary pump, cooled sample manager and column oven (Waters, Milford, MA, USA). The mass spectrometer was a Xevo G2 Q-TOF MS equipped with an electrospray ionization interface (Waters, Milford, MA, USA). The data were acquired by using MassLynx software (version 4.0, Waters, Milford, MA, USA).
Acquity uplc binary pump
Manufactured by Waters Corporation
Sourced in United States
The Acquity UPLC binary pump is a high-performance liquid chromatography (HPLC) pump designed for use with the Acquity UPLC system. The pump provides precise and accurate flow control for the separation and analysis of various compounds. It features a binary solvent delivery system that allows for the mixing of two different solvents, enabling researchers to create gradient elution profiles for their analytical methods.
Automatically generated - may contain errors
Lab products found in correlation
Masslynx software, by Waters Corporation (1 mentions)
Xevo g2 q tof ms, by Waters Corporation (1 mentions)
Hypersil gold c18 column, by Thermo Fisher Scientific (1 mentions)
Tq triple quadrupole mass spectrometer, by Waters Corporation (1 mentions)
C18 reversed phase column, by Waters Corporation (1 mentions)
Acquity uplc flr detector, by Waters Corporation (1 mentions)
3 protocols using acquity uplc binary pump
1
UPLC-MS/MS Analytical Protocol
2
Triolein Quantification by UPLC-MS/MS
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Samples of medium and cell lysate were analyzed on a Xevo TQ triple quadrupole mass spectrometer equipped with an Acquity UPLC binary pump, column heater, and a 2777 autosampler (Waters, Milford, MA). Chromatographic separation was carried out using a 2.1 mm × 50 mm, 1.9 µm Hypersil GOLD C18 column (Thermo Scientific) maintained at 60 °C. A binary solvent system composed of 10 mM ammonium formate in 40% H 2 O:60% acetonitrile (v/v, eluent A) and 90% isopropyl alcohol:10% acetonitrile (v/v, eluent B) was used for the separations. The column was initially conditioned with 90% solvent A. Immediately following injection, the solvent composition was ramped to 95% solvent B over 2.5 min. At 2.6 min, the solvent composition was returned to 90% solvent A and held for 0.4 min before the next injection. The flow rate was held constant throughout the run at 400 µL/min. A total of 5 µL of the upper pentanol phase was directly injected from the plates by setting an appropriate depth for the autosampler syringe. SRM transitions for 12 C triolein and the 13 C 21 glyceryl trioleate internal standard were acquired as described above.
3
UPLC-FLR Analysis of Ochratoxin A
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OTA was commercially purchased from Sigma Chemical Cp. (St Louis, MO, USA). Standard stock solution (1000 µg/g) was prepared in 1 mL methanol and stored at 8 °C until used. From this, five working stock solutions (2, 4, 6, 8 and 10 µg/g) were prepared in methanol and stored at 4 °C until analysed using UPLC.
UPLC-FLR ANALYSIS UPLC system consisting of Waters Acquity UPLC ® binary pump equipped with Waters Acquity UPLC ® FLR detector was used for quantification of OTA produced by isolates. For detection, the excitation and emission maxima were set at 330 and 460 nm wavelength, respectively. Chromatographic separations were carried out on a C18 reversed-phase column (2.1 × 100 mm, 1.7 µm) (Waters, USA) with isocratic programme of 57% acetonitrile (CH 3 CN), 41% water and 2% acetic acid (filtered through a PTFE syringe filter). The separation was performed at a flow rate of 0.2 mL/min with total run time of 4 min. For OTA quantification, peak height of the sample was compared with the calibration curve of the standards.
UPLC-FLR ANALYSIS UPLC system consisting of Waters Acquity UPLC ® binary pump equipped with Waters Acquity UPLC ® FLR detector was used for quantification of OTA produced by isolates. For detection, the excitation and emission maxima were set at 330 and 460 nm wavelength, respectively. Chromatographic separations were carried out on a C18 reversed-phase column (2.1 × 100 mm, 1.7 µm) (Waters, USA) with isocratic programme of 57% acetonitrile (CH 3 CN), 41% water and 2% acetic acid (filtered through a PTFE syringe filter). The separation was performed at a flow rate of 0.2 mL/min with total run time of 4 min. For OTA quantification, peak height of the sample was compared with the calibration curve of the standards.
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