Human cervical carcinoma HeLa cell line, human lung carcinoma A549 cell line and human umbilical vein endothelial HUVEC cell line were obtained from KeyGEN Biotech Co. Ltd. (Nanjing, China). All cell lines were cultured in Dulbecco's modified Eagle's medium (DMEM) containing 10% FBS, 100 μg mL-1 streptomycin and 100 U mL-1 penicillin at 37 °C in a humidified incubator containing 5% CO2 and 95% air. The medium was replenished every other day and the cells were subcultured after reaching confluence. Cell numbers were determined with a Petroff-Hausser cell counter (USA).
Huvec cell line
Manufactured by Keygen Biotech
Sourced in China
The HUVEC cell line is a primary cell culture derived from human umbilical vein endothelial cells. This cell line is commonly used in research applications to study endothelial cell biology and vascular function.
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Lab products found in correlation
2 protocols using huvec cell line
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Cell Culture Conditions for Cancer and HUVEC
2
Multicolor Cell Labeling for Co-Culture
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The HNSCC cell line FaDu, initially acquired from Suzhou Bei Na Chuanglian Biotechnology (#BNCC316798), was cultured in MEM medium supplemented with 10% (v/v) FBS (all from KeyGEN BioTECH). To facilitate monitoring during co-culture experiments, the FaDu cell line was fluorescently labeled with CellTrace™ CSFE (ThermoFisher Scientific) following the manufacturer's instructions.
Human fibroblasts (HF) were isolated as previously described [19 (link)], and cultured in a high-glucose DMEM medium supplemented with 10% (v/v) FBS (all from KeyGEN BioTECH). During co-culture experiments, the HF cell line was fluorescently labeled with CellTrace™ Far Red (ThermoFisher Scientific).
THP-1 cell line was purchased from KeyGEN BioTECH (#KG224), and cultured in RPMI-1640 media supplemented with 10% (v/v) FBS (all from KeyGEN BioTECH). The THP-1 cell line was fluorescently labeled with CellTrace™ Violet (ThermoFisher Scientific) during co-culture experiments.
The HUVEC cell line was obtained from KeyGEN BioTECH(#KG419) and cultured in high-glucose DMEM medium supplemented with 10% (v/v) FBS (all from KeyGEN BioTECH).
Fluorescently labeled cell lines were employed to monitor the different cell types during co-culture. All cultures were incubated at 37 °C in a humidified atmosphere containing 5% CO2 and sub-cultured every 2–3 days.
Human fibroblasts (HF) were isolated as previously described [19 (link)], and cultured in a high-glucose DMEM medium supplemented with 10% (v/v) FBS (all from KeyGEN BioTECH). During co-culture experiments, the HF cell line was fluorescently labeled with CellTrace™ Far Red (ThermoFisher Scientific).
THP-1 cell line was purchased from KeyGEN BioTECH (#KG224), and cultured in RPMI-1640 media supplemented with 10% (v/v) FBS (all from KeyGEN BioTECH). The THP-1 cell line was fluorescently labeled with CellTrace™ Violet (ThermoFisher Scientific) during co-culture experiments.
The HUVEC cell line was obtained from KeyGEN BioTECH(#KG419) and cultured in high-glucose DMEM medium supplemented with 10% (v/v) FBS (all from KeyGEN BioTECH).
Fluorescently labeled cell lines were employed to monitor the different cell types during co-culture. All cultures were incubated at 37 °C in a humidified atmosphere containing 5% CO2 and sub-cultured every 2–3 days.
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