PEG-CdTe QDs and dimethyl sulfoxide (DMSO) were purchased from Sigma-Aldrich (St. Louis, USA). DOX was obtained from Sigma (Wuhan, China). 1-(3-dimethylamino propyl)-3-ethyl carbon diimine hydrochloride (EDC) and N-hydroxysuccinide (sulfo-NHS) were offered by Sigma(Shanghai,China). Roswell Park Memorial Institute medium (RPMI) 1640 and fetal bovine serum (FBS) were bought from Gibco Chemical Co. (Carlsbad, CA, USA). Pierce™ bicinchoninic acid (BCA) protein assay kits, Annexin V-fluorescein isothiocyanate (FITC) apoptosis detection kits, and hematoxylin–eosin kits were provided by KeyGen Biotech Co., Ltd. (Jiangsu, China). Cell counting kit-8 (CCK-8) was obtained from Dojindo Molecular Technologies. Inc. (Nanjing, China). Monoclonal antibodies for CXCR4, SURVIVIN, Bcl-xl, cleaved caspase-3/9, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were made in Santa Cruz Biotechnology Inc. (CA, USA). All reagents were analytically pure.
Annexin 5 fluorescein isothiocyanate fitc apoptosis detection kit
Manufactured by Keygen Biotech
Sourced in China
The Annexin V-fluorescein isothiocyanate (FITC) apoptosis detection kit is a laboratory tool used to detect and quantify apoptosis, a form of programmed cell death. The kit utilizes Annexin V, a protein that binds to phosphatidylserine, a cellular membrane component exposed during apoptosis. Annexin V is conjugated with the fluorescent dye FITC, allowing for the visualization and analysis of apoptotic cells using flow cytometry or fluorescence microscopy.
Automatically generated - may contain errors
Lab products found in correlation
Facscalibur, by BD (7 mentions)
Facscalibur flow cytometer, by BD (5 mentions)
Cell cycle detection kit, by Keygen Biotech (4 mentions)
Facscan instrument, by BD (3 mentions)
Facscan flow cytometer, by BD (3 mentions)
Gapdh, by Santa Cruz Biotechnology (2 mentions)
Pi rnase staining kits, by MultiSciences Biotech (2 mentions)
Facsdiva, by BD (2 mentions)
Flow cytometry, by BD (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Triton x 100, by Merck Group (2 mentions)
Methyl thiazolyl tetrazolium (mtt), by Merck Group (2 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Bcl xl, by Santa Cruz Biotechnology (1 mentions)
Propidium iodide, by Merck Group (1 mentions)
Dcfh da, by Beyotime (1 mentions)
Facsaria flow cytometer, by BD (1 mentions)
Fascdiva 4, by BD (1 mentions)
Inverted fluorescence microscope, by Nikon (1 mentions)
Caspase 3 activity assay kit, by Beyotime (1 mentions)
59 protocols using annexin 5 fluorescein isothiocyanate fitc apoptosis detection kit
1
PEG-CdTe QDs-DMSO Cytotoxicity Assay
2
Cell Cycle and Apoptosis Analysis
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Cells were harvested directly or 48 h after siRNA transient transfection and washed with ice-cold phosphate-buffered saline (PBS). The PI/RNase staining kits (Multisciences, Hangzhou, China) and annexin V-fluorescein isothiocyanate (FITC) apoptosis detection kits (KeyGEN Biotech, Nanjing, China) were used to detect cell cycle and apoptosis in a FACScan instrument (Becton Dickinson,, Mountain View, CA, USA), respectively.
3
Annexin V Apoptosis Assay
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Cells were harvested directly or 48 h after siRNA transient transfection and washed with ice-cold phosphate-buffered saline (PBS). The annexin V-fluorescein isothiocyanate (FITC) apoptosis detection kits (KeyGEN Biotech, Nanjing, China) was used to detect apoptosis in a FACScan instrument (Becton Dickinson,, Mountain View, CA, USA), respectively.
4
Cell Cycle and Apoptosis Analysis
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Cells were harvested directly or 48 h after siRNA transient transfection and washed with ice-cold phosphate-buffered saline (PBS). The PI/RNase staining kits (Multisciences, Hangzhou, China) and annexin V-fluorescein isothiocyanate (FITC) apoptosis detection kits (KeyGEN Biotech, Nanjing, China) were used to detect cell cycle and apoptosis in a FACScan instrument (Becton Dickinson,, Mountain View, CA, USA), respectively.
5
HVEM-shRNA Modulates OVCAR3 Cell Cycle and Apoptosis
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OVCAR3 cells cultured in six-well plates were infected with HVEM-shRNA or control virus. At 48 h after infection, cells were harvested and washed with ice-cold phosphate-buffered saline (PBS). For cell cycle analysis, cells were fixed with cold 70% ethanol at −20°C overnight, washed twice with ice-cold PBS, and stained with propidium iodide (PI; 20 µg/ml; Sigma-Aldrich) and RNase (100 µg/ml; Sigma-Aldrich) in the dark for 20 min. For cell apoptosis analysis, cells were stained with annexin V-fluorescein isothiocyanate (FITC) apoptosis detection kits (KeyGEN Biotech, Nanjing, China). Cell cycle distribution and cell apoptosis were analyzed using a flow cytometer (BD Biosciences, Franklin Lakes, NJ, USA).
6
Annexin-V-FITC Apoptosis Assay
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Cells were harvested, washed with ice-cold PBS, and stained with Annexin-V-fluorescein isothiocyanate (FITC) apoptosis detection kits (KeyGEN Biotech, Nanjing, P.R. China). Cell apoptosis was analyzed in a flow cytometer (BD Biosciences).
7
Quantifying Cellular Pyroptosis by Flow Cytometry
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After washing with ice-cold PBS, cells were incubated with 2’,7’-Dichlorodihydrofluorescein diacetate (DCFH-DA) (10 µM, Beyotime, Shanghai, China) for 20 min at 37°C. Cells were stained with either annexin V-fluorescein isothiocyanate (FITC) apoptosis detection kits (KeyGEN Biotech, Nanjing, China), or caspase-1 (#9122, Bio-Rad Laboratories, Hercules, CA, USA) and propidium iodide (PI) (P3566, Thermo Fisher Scientific, Waltham, MA, USA). Cell pyroptosis (positive for caspase-1and PI) was analyzed using flow cytometry (BD Biosciences, Franklin Lakes, NJ, USA).
8
Apoptosis Analysis by Flow Cytometry
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Cells were harvested, washed with ice-cold PBS, and stained with annexin V-fluorescein isothiocyanate (FITC) apoptosis detection kits (KeyGEN Biotech, Nanjing, China). Cell apoptosis was analyzed in a flow cytometer (BD Biosciences).
9
Annexin-V and PI Apoptosis Assay
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The percentage of normal nonapoptotic cells and apoptotic cells was measured by double supravital staining with Annexin-V and propidium iodide (PI) following manufacturer's instruction, using an annexin V-fluorescein isothiocyanate (FITC) apoptosis detection kit (KeyGen, Nanjing, China). Briefly, cells were centrifuged, washed with cold phosphate-buffered saline twice, and then resuspended in 500 μl binding buffer. FITC-conjugated Annexin V (5 μl) and PI (5 μl) were added to each sample, and the mixture was incubated at room temperature in the dark for 15 min. The cells were then subjected to fluorescence-activated cell sorting (FACS) analysis (BD Facscalibur, BD Biosciences, Franklin Lakes, NJ, USA). During all FACS analyses, 105 events for each sample were analyzed. The percentage of apoptotic cells in each group was determined.
10
Annexin V-FITC Apoptosis Assay
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Cells were collected and treated with trypsin without ethylenediaminetetraacetate (EDTA) to obtain a single cell suspension. Subsequently, the Annexin V-fluorescein isothiocyanate (FITC) apoptosis detection kit (KGA107, KeyGEN BioTECH Corp., Ltd., Jiangsu, China) was used to detect cell apoptosis. The collected cells were suspended with 500 μL of binding buffer. After adding 5 μL of Annexin V-FITC and 5 μL of propidium iodide (PI), cells were reacted for 15 min at room temperature in the dark and tested by flow cytometry within 1 h.
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