Klebsiella pneumoniae atcc 13883

Urine samples showing a colony count more than 10⁴ cfu/mL were considered to be positive for UTI. UTI isolates were identified following standard biochemical tests. Results were not considered for more than two clinical isolates obtained from the same patient and the sample was considered to be contaminated. No-growth plates were considered as sterile.
For positive urine cultures, identifications were done using automated system Microscan (Walkaway 40 SI, Siemens Healthcare Diagnostics, Sacramento, CA). Panels used for Gram-negative bacteria (NC34 and NC53) and for Gram-positive bacteria (PC21) were obtained from Siemens Healthcare Diagnostics (Sacramento, CA). For confirmation, further biochemical tests were done for both Gram-positive and Gram-negative isolates (API E20, API strep, and API staph) supplied by bioMérieux, (Durham, NC, USA).
QC strains (Escherichia coli ATCC 25922, Klebsiella pneumoniae ATCC 13883, and Candida albicans ATCC 10231) were supplied by American Type Culture Collection (ATCC) (Manassas, VA).

Brevibacterium sp. 7002-073 (GenBank accession No: KY770501.1) was isolated from the cyanobacterium Synechococcus sp. PCC7002, which was collected off the coast of China in the Yellow Sea near Qingdao, China. The multi-drug resistant (MDR) bacterial strains Staphylococcus aureus CCARM 3090, Escherichia coli CCARM 1009, Enterococcus faecalis CCARM 5172, Enterococcus faecium CCARM 5203, and Salmonella typhimurium CCARM 8250 were bought from the Culture Collection of Antimicrobial Resistant Microbes (Seoul Women’s University of Korea), and Acinetobacter baumannii ATCC 19606 and Klebsiella pneumoniae ATCC 13883 were bought from the American Type Culture Collection. The plasmids used in this study are listed in Table S5.
The Brevibacterium sp. 7002-073 strain was routinely cultured at 30 °C in the brain heart infusion broth (BHI) liquid medium or on BHI agar plate. BHI medium supplemented with 2% glycine and 10% sodium succinate was used for electro-competent cell preparation. The Brevibacterium sp. 7002-073 transformants harboring plasmid were incubated in the BHI medium with thiostrepton (5 µg/mL). The fermentation medium (M8) consists of 2% soluble starch, 1% glucose, 0.2% meat extract, 0.2% yeast extract, 0.3% CaCO₃ and 0.4% casein (pH = 7.0). The MDR bacterial strains were cultured in Luria-Bertani (LB) medium at 37 °C.