Gas chromatography with flame ionization detection (GC-FID) was used to determine the fatty acid side chains in the purified MEL after derivatization to the corresponding methyl esters. First, 10 mg of MEL were mixed with 1.5 ml of 5% acetyl chloride in methanol and vortexed for 1 min, before the samples were incubated at 100°C for 1.5 h and left to cool. Then, 1.5 ml of water and 2 ml of hexane containing an internal standard (C15-ME) were added and the samples were vortexed for 1 min. The phases were separated by centrifugation (5000 rpm, 10 min) and the hexane phase containing the methyl esters transferred into GC vials. GC analysis was performed on an Agilent 7890A GC-FID system with a SPB-PUFA capillary column (30 m × 0.32 mm, df = 0.2 μm; Sigma-Aldrich, USA). Helium at 50 ml/min was used as carrier gas and the temperature gradient ranged from 60°C to 220°C. Injection volume was 1 μl. A Supelco 37 Component FAME Mix (Sigma-Aldrich, USA), containing linear saturated and unsaturated fatty acids, was used as the reference standard.
Agilent 7890a gc fid system
Manufactured by Agilent Technologies
Sourced in United States
The Agilent 7890A GC-FID system is a gas chromatography instrument with a flame ionization detector (FID). It is designed for the separation, identification, and quantification of a wide range of organic compounds in various sample matrices.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using agilent 7890a gc fid system
1
Fatty Acid Profiling of Purified Mannosylerythritol Lipids
2
Analysis of SEO Volatile Aroma Components
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The volatile aroma components of SEO were measured based on a previous study [4 (link)]. Briefly, 1 μL of essential oil was injected at a split ratio of 1:50 into an Agilent 7890A GC-FID system (Agilent J&W, Santa Clara, CA, USA). The volatile aroma components were separated using an Agilent J&W DB-Wax column (60 m × 0.25 mm i.d., 0.25-μm film thickness) under a helium stream at a flow rate of 32 cm/s. The initial oven temperature was 40 °C, which was maintained for 2 min, then increased to 200 °C at a rate of 2 °C/min, and finally held for 38 min. The GC injector and FID were set at 250 °C. Aroma components were identified based on linear retention indices (RIs) upon measurement of the homologous series of C7–C30 alkanes, followed by comparison of the MS patterns of the peak with MS data from the National Institute of Standards and Technology (NIST) MS Library, Version 2008, and MS patterns of authentic standards. The MS patterns of the peaks were acquired using an Agilent 5975C MS system under the following conditions: both ion source and interface temperatures were set at 230 °C, electron impact ionization at 70 eV, and mass acquisition range of 29–450 amu. The composition was expressed as the relative concentration of the FID peak area response. All analyses were performed in triplicate.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!