Animals were anesthetized in 0.2 mg/ml tricaine methanesulphonate (MS222) during the operations. Operations on large wild collected animals (3-10 cm in length) were conducted as described previously (Auger et al., 2010 (link)), and they were maintained in natural running seawater at ambient temperature during regeneration. Ciona are sensitive to surgical procedures that remove large parts of the bodies, which can evoke a stress response earmarked by a large increase in the number of orange pigment cells throughout the body and particularly the pharyngeal region (Parrinello et al, 2010 ). The stress response could occur even when animals were carefully removed from their normal attachments in nature or laboratory culture. To minimize this response, whenever possible the operations were carried out while animals were attached to Petri dishes, which also increased the survival rate. Operations were carried out using sharp tungsten needles, fine forceps, and straight bladed sharp microcautery scissors (Fine Science Tools Inc., Foster City, CA).
Straight bladed sharp microcautery scissors
Manufactured by Fine Science Tools
Sourced in United States
Straight bladed sharp microcautery scissors are a precision surgical instrument designed for controlled tissue cutting and coagulation during delicate procedures. They feature sharp, fine-tipped blades for accurate dissection and hemostasis.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using straight bladed sharp microcautery scissors
1
Surgical Procedures for Ciona Regeneration
2
Regeneration in Ciona Ascidians
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Animals were anesthetized in 0.2 mg/mL tricaine methanesulfonate (MS222) during the operations. Operations on large wild collected animals (3−10 cm in length) were conducted as described previously (Auger et al. 2010 ), and they were maintained in natural running seawater at ambient temperature during regeneration. Ciona are sensitive to surgical procedures that remove large parts of the bodies, which can evoke a stress response earmarked by a large increase in the number of orange pigment cells throughout the body and particularly the pharyngeal region (Parrinello et al. 2010 ). The stress response could occur even when animals were carefully removed from their normal attachments in nature or laboratory culture. To minimize this response, whenever possible the operations were carried out while animals were attached to Petri dishes, which also increased the survival rate. Operations were carried out using sharp tungsten needles, fine forceps, and straight bladed sharp microcautery scissors (Fine Science Tools Inc., Foster City, CA, USA).
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