Anti rpb2

The following primary antibodies were used in this work: anti-Lsm11, anti-hnRNP UL1, anti-RPB2 (Abcam), anti-ZN473 (Abgent), anti-symplekin, anti-V5, anti-FUS Ab1, anti-FUS Ab2, anti-TAF15 (Bethyl Laboratories), anti-SLBP, anti-Lsm10, anti-lamin A/C, anti-HA, anti-Cyclin B1, anti-GADPH, antiH2A, anti-H2B, anti-H2A.Z, anti-H4 (Santa Cruz Biotechnology), anti-β-actin (MP Biomedicals), anti-Maltose Binding Protein, anti-FLAG (Sigma Aldrich), Y12 monoclonal antibody (recognizing SmB/B’, SmD1) as described in (61 (link)). The following secondary antibodies were used: goat anti-rabbit IgG-HRP, donkey anti-goat IgG-HRP, goat anti-mouse IgG-HRP (Santa Cruz Biotechnology).
A polyclonal rabbit anti-FUS antibody was prepared as follows: a cDNA fragment, amplified by PCR, encoding the first 286 amino acids of FUS was cloned between the EcoRI and XhoI sites of pET28a. The recombinant protein was expressed in BL21(DE3) Codon Plus RIPL and purified under denaturing conditions over Ni-NTA beads according to the manufacturer's instructions. The purified protein was dialyzed against PBS, and rabbits were immunized with the purified protein in combination with GERBU Adjuvant LQ. Primer sequences used for cloning and are available on request.