Seawater and pore water samples were collected from the vicinity of seagrass meadows at Sant’ Andrea, Elba, Italy, in July 2017 to determine the δ-13C of dissolved inorganic carbon (DIC). Seawater was sampled at the surface from a boat, whereas pore water was sampled at 15-cm sediment depth with a steel lance. Samples were drawn into 20-ml plastic syringes; 6 ml of each was fixed with 100 μl of 300 mM ZnCl2 and stored at 4°C until processing. δ-13C was measured with a Finnigan MAT 252 gas isotope ratio mass spectrometer with Gasbench II (Thermo Scientific), using Solnhofen limestone as a standard and 8 technical replicates per sample.
Mat 252
Manufactured by Thermo Fisher Scientific
The MAT 252 is a magnetic sector mass spectrometer designed for high-precision isotope ratio analysis. It features a dual-inlet system for sample introduction and a multi-collector detection system for simultaneous measurement of multiple isotopes. The MAT 252 is capable of providing accurate and reproducible isotope ratio data for a variety of applications, including geological, environmental, and biomedical research.
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Lab products found in correlation
3 protocols using mat 252
1
Stable Isotopic Analysis of Coastal DIC
2
Paleosol n-Alkane Isotopes and PAHs
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Ten of these samples had already been analyzed for n-alkane carbon isotopes in a previous study (23 ), and new analyses were conducted to investigate PAHs in all samples. Paleosol sediments were cleaned, freeze-dried, and powdered. Lipids were extracted from sediments using Soxhlet extraction and separated via accelerated solvent extraction as per Magill et al. (57 ). PAHs and n-alkanes were quantified and identified via GC-MS (Hewlett-Packard 6890 GC and Hewlett-Packard 5973 MS). Carbon isotopes of n-alkanes were measured using an irm-GCMS (Varian 3400 GC connected to Thermo MAT 252), and standard mean error for each measurement was 0.3‰ or less (1σ) (58 ). Carbon isotope values are reported in per mil (‰) delta notation normalized to the international standard Vienna Pee Dee Belemnite (VPDB) using certified reference materials (Schimmelman A6). Delta notation is defined as δ13C = ([13C/12C]sample/[13C/12C]VPDB − 1) × 1000. To evaluate potential laboratory contamination, blanks were run for each processing step, and molecules of interest in this study were below detection. Detailed methods are available in SI Appendix, Extended Methods .
3
Stable Isotope Analysis of Horseshoe Lake Sediments
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Sediment cores and water samples were collected from Horseshoe Lake in 2012 and 2013 CE. The age-model and associated 95% confidence intervals for the interpolated ages were generated with CLAM (v. 2.2) using eight calibrated radiocarbon and 14 210Pb dates20 (link). The δ18O of authigenic calcite isolated from the Horseshoe Lake sediment archive was measured using a Thermo Gas Bench II connected under continuous flow to a Thermo MAT 252 stable isotope ratio mass spectrometer at Indianapolis University–Purdue University in Indianapolis (IUPUI). All lake sediment calcite δ18O results are reported in standard delta notation relative to Vienna Pee Dee Belemnite (VPDB) in ‰. Analytical precision, based on repeated measurements of standards, was 0.08 ‰ for calcite δ18O20 (link). Regional surface water oxygen and hydrogen (δ2H) isotopes were analyzed at IUPUI using a Picarro L2130-i Analyzer44 (link). Final values were corrected to Vienna Standard Mean Ocean Water (VSMOW) and are in ‰. Analytical precision was 0.02‰ and 0.30‰ for water δ18O and δ2H, respectively.
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