Ese quant lr3 lateral flow system

Manufactured by Qiagen

Sourced in Germany

The ESE Quant LR3 is a lateral flow system designed for quantitative analysis. It provides a platform for rapid, sensitive, and accurate measurement of analytes in various sample types.

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Lab products found in correlation

Hf000mc100, by Merck Group (2 mentions) Absorbent pad, by Cytiva (2 mentions) Hepes, by Thermo Fisher Scientific (1 mentions) Gfcp001000, by Merck Group (1 mentions) Goat anti mouse igg, by MyBioSource (1 mentions) Gold 3 chloride hydrate haucl4, by Merck Group (1 mentions) Histamine dihydrochloride, by Merck Group (1 mentions) Recombinant protein a g, by Thermo Fisher Scientific (1 mentions) Unisart cn95, by Sartorius (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions)

6 protocols using ese quant lr3 lateral flow system

Immunoassay Development Using Tetraspanins

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Anti-tetraspanin antibodies were purchased at Immunostep (Salamanca, Spain): anti-CD9 (clone VJ1/20), anti-CD63 (clone Tea 3/18), and anti-CD147 (clone VJ1/9). Anti-mouse IgG, bovine serum albumin (BSA), 1-ethyl-3-[3-dimethylaminopropyl]-carbodiimide hydrochloride (EDC), N-hydroxysuccinimide (NHS) were provided by Sigma-Aldrich (Madrid, Spain). HEPES was purchased from Fisher Scientific.
Some materials for strips were provided by Millipore (Darmstadt, Germany): glass fibre membrane (GFCP001000) used as sample pad, nitrocellulose membrane (HF07504XSS) and backing cards (HF000MC100). Absorbent pad was purchased from Whatman (Piscataway, NJ, USA). The sample buffer used was 10 mM HEPES pH 7.4 with 0.05% Tween-20 and 1% BSA.
To prepare the detection and control lines, an IsoFlow reagent dispensing system was used. A guillotine Fellowes Gamma (Madrid, Spain) was used to cut the strips. In order to quantify the intensity of the test line using reflectance measurements, a portable strip reader ESE Quant LR3 lateral flow system (Qiagen Inc., Hilden, Germany) was used.

Moyano A., Serrano-Pertierra E., Duque J.M., Ramos V., Teruel-Barandiarán E., Fernández-Sánchez M.T., Salvador M., Martínez-García J.C., Sánchez L., García-Flórez L., Rivas M, & Blanco-López M.D. (2021). Magnetic Lateral Flow Immunoassay for Small Extracellular Vesicles Quantification: Application to Colorectal Cancer Biomarker Detection. Sensors (Basel, Switzerland), 21(11), 3756.

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Quantifying Test Line Color Intensity

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Reflectance measurements were performed to quantify the color intensity of the test line. For this, a portable strip reader ESE-Quant LR3 lateral flow system (Qiagen Inc., Germany) was used. This equipment contains two channels (LED excitation and photodiode detection) in order to scan the strip by illuminating it with a light beam. Then, a confocal detector measures the attenuation from the surface of the strip, registering the signal and converts it into an electrical signal, which is related to the amount of analyte at the test lines. The unit provided by this device is mm·mV, resulting from integrating the electrical signal (mV) across the width of the test line (mm).

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Quantitative Detection of E. coli O157:H7

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Mouse monoclonal antibodies to Escherichia coli O157:H7 (MBS568290 and MBS568193) were purchased from Mybiosource (San Diego, CA, USA).
Goat anti-mouse IgG, N-hydroxysuccinimide (NHS), 1-ethyl-3-[3-dimethylaminopropyl]–carbodiimide hydrochloride (EDC), 2-(N-morpholino)ethanesulfonic acid (MES), bovine serum albumin (BSA), phosphate-buffered saline (PBS), silver nitrate (AgNO₃), hydroquinone (C₆H₄-1,4-(OH)₂), gold(III) chloride hydrate (HAuCl₄), and sodium citrate tribasic dihydrate (C₆H₅Na₃O₇·2H₂O) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Sodium dodecyl sulfate (SDS) (C1₂H₂5NaO₄S) was purchased from PanReac AppliChem (Barcelona, Spain).
HS-C₂H₄-CONH-PEG-C₃H₆-COOH, MW = 5000 g mol⁻¹ (4900 Da), and CH₃O-PEG-NH₂, MW = 725–850 Da were purchased from RAPP POLYMER (Tuebingen, Germany). All the reagents were prepared using Milli-Q ultrapure water (resistivity 18.2 MΩ·cm at 25 °C), unless otherwise stated.
An IsoFlow reagent dispensing system (ImageneTechnology, USA) was used to dispense the detection lines (dispense rate 0.100μL mm⁻¹) and the strips were cut with a guillotine Fellowes Gamma (Spain).
A portable strip reader ESE Quant LR3 lateral flow system (Qiagen Inc., Germany) was used to quantify the intensity of the test line by reflectance measurements.

Bazsefidpar S., Serrano-Pertierra E., Gutiérrez G., Calvo A.S., Matos M, & Blanco-López M.C. (2023). Rapid and sensitive detection of E. coli O157:H7 by lateral flow immunoassay and silver enhancement. Mikrochimica Acta, 190(7), 264.

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Quantifying Magnetic Nanoparticle Absorbance

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Analysis of absorbance of magnetic nanoparticles with various coatings was achieved with a UV–Vis spectrophotometer (PG Instrument, LTD) together with UV/Win Spectrophotometer Software. An IsoFlow reagent dispensing system (Imagene Technology, Hanover, USA) was used to dispense the detection lines (dispense rate: 0.100 μL/mm) and the strips were cut with a guillotine Fellowes Gamma (Madrid, Spain). A portable strip reader ESE Quant LR3 lateral flow system (Qiagen Inc., Hilden, Germany) was used to quantify the intensity of the test line by means of reflectance measurements.

Wang B., Moyano A., Duque J.M., Sánchez L., García-Santos G., Flórez L.J., Serrano-Pertierra E, & Blanco-López M.D. (2022). Nanozyme-Based Lateral Flow Immunoassay (LFIA) for Extracellular Vesicle Detection. Biosensors, 12(7), 490.

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Quantitative Lateral Flow Assay Analysis

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A portable strip reader ESE-Quant LR3 lateral flow system (Qiagen Inc., Germany) was used to quantify the colour intensity of the test line by reflectance measurements. The optical reader analyses the reflectance at the control and test line by using two channels (LED excitation and photodiode detection).
The reader scans the strip by illuminating it with a light beam and then measures the attenuation from the surface of the strip through a confocal detector. The detector registers the signal and converts it into an electrical signal that is related to the amount of analyte at the control and test lines. The device provides values in units of mm•mV, resulting from integrating the electrical signal (mV) across the width of the test line (mm).

Moyano A., Salvador M., Martínez-García J.C., Socoliuc V., Vékás L., Peddis D., Alvarez M.A., Fernández M., Rivas M, & Blanco-López M.C. (2019). Magnetic immunochromatographic test for histamine detection in wine. Analytical and bioanalytical chemistry, 411(25).

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Lateral Flow Assay for Histamine Detection

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Mouse histamine monoclonal antibody (MBS2025715) and histamine-BSA conjugate antigen (MBS358205) were purchased from Mybiosource. Anti-mouse IgG, Bovine Serum Albumin (BSA), 1ethyl-3-[3-dimethylaminopropyl]-carbodiimide hydrochloride (EDC), N-hydroxysuccinimide (NHS), 2-(N-Morpholino)ethanesulfonic acid (MES) and histamine dihydrochloride were provided by Sigma-Aldrich (Spain). Recombinant protein A/G was purchased at Thermo-Scientific (Massachusetts, USA).
Gold nanoparticles of size 40 nm were purchased from BB International (UK). Disposable 0.45 µm PVDF filters were purchased from GE Healthcare Life Sciences.
Glass fibre membrane (GFCP001000) used as sample pad and backing cards (HF000MC100) were purchased from Millipore (Germany). Other materials used were nitrocellulose membranes (UniSart CN95, Sartorius, Spain) and absorbent pads (Whatman, USA). Based on previous results, the sample buffer consisted of 10 mM Phosphate-Buffer (PB) pH 7.4 with 0.5% Tween-20 and 1% BSA.
An IsoFlow reagent dispensing system (Imagene Technology, USA) was used to dispense the detection lines (dispense rate 0.100 µL/mm) and the strips were cut with a guillotine Fellowes Gamma (Spain).
A portable strip reader ESE Quant LR3 lateral flow system (Qiagen Inc., Germany) was used to quantify the intensity of the test line by reflectance measurements.

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