Reagents were purchased from Fisher Scientific (Hampton, NH) or Sigma (St. Louis, MO) unless otherwise specified. All reagents were validated by the manufacturer and/or have been previously cited in the literature. Antibodies used were: anti-α-Tubulin (T9026; Sigma, St. Louis, MO), anti-pFAK397 (3283; Cell Signalling Technology, Danvers, MA), anti-pPaxillin118 (44–722 G; ThermoFisher Scientific, Waltham, MA), anti-cadherin-11 (71–7600; ThermoFisher Scientific, Waltham, MA), anti–phospho-p44/42 ERK (Thr202/Tyr204; 4370; Cell Signalling Technology, Danvers, MA), anti-p44/42 ERK (9102; Cell Signalling Technology, Danvers, MA), anti-Ki67 (ab15580; Abcam, Cambridge, UK), AlexaFluor 488 anti-rabbit secondary (A-11,008; ThermoFisher Scientific, Waltham, MA), and AlexaFluor 647 anti-rabbit secondary (A-21,244; ThermoFisher Scientific, Waltham, MA). ECM substrates used were: Collagen I (CB-40,236; Fisher Scientific, Hampton, NH). Inhibitors used were: Amiodarone hydrochloride (40–955–0; Tocris Bioscience, Minneapolis, MN), Carvedilol (C3993, Sigma, St. Louis, MO), Imipramine hydrochloride (I7379; Sigma, St. Louis, MO), and Thioridazine hydrochloride (T9025, Sigma, St. Louis, MO).
Anti pfak397
Manufactured by Cell Signaling Technology
Anti-pFAK397 is a laboratory reagent that detects phosphorylated focal adhesion kinase (pFAK) at serine 397. It is used to identify and quantify the presence of pFAK397 in biological samples through various analytical techniques.
Automatically generated - may contain errors
Lab products found in correlation
Collagen 1, by Thermo Fisher Scientific (1 mentions)
Amiodarone hydrochloride, by Bio-Techne (1 mentions)
Carvedilol, by Merck Group (1 mentions)
Anti ki67, by Abcam (1 mentions)
Anti α tubulin, by Merck Group (1 mentions)
Imipramine hydrochloride, by Merck Group (1 mentions)
Anti p44 42 erk, by Cell Signaling Technology (1 mentions)
Anti cadherin 11, by Thermo Fisher Scientific (1 mentions)
Thioridazine hydrochloride, by Merck Group (1 mentions)
Anti integrin β3, by Cell Signaling Technology (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Beyotime (1 mentions)
Anti cd68, by BioLegend (1 mentions)
Anti p src, by Cell Signaling Technology (1 mentions)
Anti integrin β1, by Cell Signaling Technology (1 mentions)
Lactate dehydrogenase ldh assay, by Beyotime (1 mentions)
Anti β actin, by Cell Signaling Technology (1 mentions)
Pf 573228, by Selleck Chemicals (1 mentions)
Anti fak, by Cell Signaling Technology (1 mentions)
Anti src, by Cell Signaling Technology (1 mentions)
Pf 431396, by Merck Group (1 mentions)
2 protocols using anti pfak397
1
Cellular Adhesion and Signaling Assay
2
Inhibition of Pyk2 and Rac1 Signaling
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NaHS was administered instead of H2S. NaHS and the Pyk2 inhibitor (PF431396) were obtained from Sigma-Aldrich; The FAK specific inhibitor (PF573228), the Src inhibitor (PP2), and the Rac1 inhibitor (NSC23766) were purchased from Selleck Chemicals. CES-siRNA (sc-142618) was obtained from Santa Cruz. Rhodamine was obtain from Cytoskeleton, and DAPI from Beyotime; Following antibodies were used: anti-Rac, anti-Fak, anti-p-FAK397, anti-p-FAK925, anti-Src, anti-p-Src, anti-β-actin, anti-Integrin β1, anti-Integrin β3, anti-Cavenolin-1,anti-p-Pyk2 (Cell Signaling); anti-CSE (Santa Cruz); anti-Integrin β1-FITC, anti-Galectin-3 (eBbioscience); anti-CD68 (Biolegend); Lactate dehydrogenase (LDH) assay from BeyotimeInstitute of Biotechnology.
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