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Elisa kits for human il 6

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ELISA kits for human IL-6 are laboratory tools used to measure the concentration of interleukin-6 (IL-6), a cytokine, in human samples. These kits provide a quantitative assessment of IL-6 levels through an enzyme-linked immunosorbent assay (ELISA) technique.

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Lab products found in correlation

Tnf α, by R&D Systems (2 mentions) Htra2, by R&D Systems (1 mentions) Il 1ra, by R&D Systems (1 mentions) Il 17a, by R&D Systems (1 mentions) Il 1β, by R&D Systems (1 mentions) Il 10, by R&D Systems (1 mentions) Polymyxin b, by Merck Group (1 mentions)

Close spelling variants of this product

ELISA kit for human IL-6 Quantikine Elisa Kit for human IL-6 ELISA kit for IL-6 ELISA kits for IL-6 Human IL-6 ELISA kit IL-6 kits Human IL-6 Human kits ELISAs

4 protocols using elisa kits for human il 6

1

Cytokine Quantification in Samples

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ELISA kits for human IL-6 (R&D Systems), IL-8 (R&D Systems), CCL2 (R&D Systems), TNFα (R&D Systems), and HtrA2 (R&D System) were used to measure cytokine concentration in culture supernatants and SFs.
Jeong G.H., Nam M.K., Hur W., Heo S., Lee S., Choi E., Park J.H., Park Y., Kim W.U., Rhim H, & Yoo S.A. (2023). Role of high-temperature requirement serine protease A 2 in rheumatoid inflammation. Arthritis Research & Therapy, 25, 96.
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2

Quantification of Cytokine Profiles

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Cytokine production was quantified in supernatants using commercial ELISA kits for human IL-6, TNFα, IL-1β, IL-10, IL-1RA (R&D Systems); mouse IL-6, IFNγ, (BD Biosciences); TNFα, IL-10 (Invitrogen); and IL-17A (R&D Systems), following manufacturer’s instructions.
Martín-Cruz L., Angelina A., Baydemir I., Bulut Ö., Subiza J.L., Netea M.G., Domínguez-Andrés J, & Palomares O. (2022). Candida albicans V132 induces trained immunity and enhances the responses triggered by the polybacterial vaccine MV140 for genitourinary tract infections. Frontiers in Immunology, 13, 1066383.
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3

Cytokine Measurement by ELISA

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Cytokine production was measured in supernatants using commercial sandwich ELISA kits for human IL‐6, tumor necrosis factor‐α (TNF‐α), IL‐1β and IL‐1Ra (R&D Systems), in accordance with the manufacturer's instructions.
Mourits V.P., Arts R.J., Novakovic B., Matzaraki V., de Bree L.C., Koeken V.A., Moorlag S.J., van Puffelen J.H., Groh L., van der Heijden C.D., Keating S.T., Netea M.G., Oosting M, & Joosten L.A. (2019). The role of Toll‐like receptor 10 in modulation of trained immunity. Immunology, 159(3), 289-297.
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4

Quantification of IL-6 in RA-FLS Cells

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RA-FLS were seeded into 6-well plates (1 × 105 cells/mL) with medium containing 10% FBS and incubated overnight. Thereafter, cells were cultured with or without sCD14 (500 ng/mL) for 24 h, followed by quantification of IL-6 in supernatants by ELISA kits for human IL-6 (R&D Systems), as per the manufacturer’s protocol. Stimulation with sCD14 was also carried out in the presence of 1 μg/mL of polymyxin B (Sigma-Aldrich, St. Louis, MO, USA).
Ichise Y., Saegusa J., Tanaka-Natsui S., Naka I., Hayashi S., Kuroda R, & Morinobu A. (2020). Soluble CD14 Induces Pro-inflammatory Cytokines in Rheumatoid Arthritis Fibroblast-Like Synovial Cells via Toll-Like Receptor 4. Cells, 9(7), 1689.
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