Fitc conjugated anti cxcr4

Manufactured by BD

Sourced in United States

FITC-conjugated anti-CXCR4 is a fluorescently labeled antibody that specifically binds to the CXCR4 chemokine receptor. CXCR4 is a G protein-coupled receptor that plays a role in cell migration and homing. This product can be used to detect and analyze CXCR4-expressing cells in various research applications.

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Lab products found in correlation

Pe conjugated anti igd, by BD (1 mentions) Anti cd86, by BD (1 mentions) Anti igm, by BD (1 mentions) Anti igm, by Thermo Fisher Scientific (1 mentions) Sytox blue, by Thermo Fisher Scientific (1 mentions) Anti cd138, by BD (1 mentions) Anti cd23, by Thermo Fisher Scientific (1 mentions) Pecy7 conjugated anti cd21, by BioLegend (1 mentions) Anti gl7, by BD (1 mentions) Brilliant violet 421 conjugated anti cd138, by BioLegend (1 mentions) Anti cd95, by BD (1 mentions) Apc conjugated anti b220, by BD (1 mentions) Anti igg1, by Thermo Fisher Scientific (1 mentions) Anti cd138, by BioLegend (1 mentions) Facscanto 2, by BD (1 mentions) Facscanto 2 flow cytometer, by BD (1 mentions)

2 protocols using fitc conjugated anti cxcr4

Comprehensive Flow Cytometry Analysis of Murine Splenocytes

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Flow cytometry analysis of spleen cell suspensions was performed using the following fluorescent-labeled anti–mouse antibodies: APC-conjugated anti-B220 (BD Biosciences #553092), anti-CD138 (BD Biosciences #558626) and anti-IgM (eBioscience #17–5790-82); PE-conjugated anti-IgD (BD Biosciences #558597), anti-CD23 (eBioscience #5010271) and anti-CD95 (BD Biosciences #554258); PECy7-conjugated anti-CD21 (BioLegend #123420), anti-CD95 (BD Biosciences #557653), anti-GL7 (BD Biosciences # 561530) and anti-CD86 (BD Biosciences #560582); PEVio770-conjugated anti-B220 (Miltenyi Biotec #130–102-308); FITC-conjugated anti-CXCR4 (BD Biosciences #551967) and anti-IgG1 (ebioscience #11–4011-85); Brilliant Violet 421-conjugated anti-CD138 (BioLegend #142507); PerCP-Cy5.5-conjugated anti-GL7 (BioLegend #144610), anti-CD138 (BioLegend #142510) and anti-IgM (BD Biosciences #550881), APC-Cy7-conjugated anti-CD19 (ebioscience #47–0193-82), AlexaFluor647-conjugated anti-BLIMP1 (BD Biosciences #563643). Sytox blue (ThermoFisher Scientific) or DAPI was used for the exclusion of dead cells. Data was acquired on a BD FACSCanto II (BD Biosciences) and analyzed using FlowJo v10.1 software (TreeStar).

Dominguez P.M., Ghamlouch H., Rosikiewicz W., Kumar P., Béguelin W., Fontán L., Rivas M.A., Pawlikowska P., Armand M., Mouly E., Torres-Martin M., Doane A.S., Calvo Fernandez M.T., Durant M., Della-Valle V., Teater M., Cimmino L., Droin N., Tadros S., Motanagh S., Shih A.H., Rubin M.A., Tam W., Aifantis I., Levine R.L., Elemento O., Inghirami G., Green M.R., Figueroa M.E., Bernard O.A., Aoufouchi S., Li S., Shaknovich R, & Melnick A.M. (2018). TET2 deficiency causes germinal center hyperplasia, impairs plasma cell differentiation and promotes B-cell lymphomagenesis. Cancer discovery, 8(12), 1632-1653.

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Analyzing Surface and Cytoplasmic CXCR4/CXCR7 Expression

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The cells were incubated with fluorescein isothiocyanate (FITC)-, phycoerythrin (PE)-, or allophycocyanin (APC)-conjugated monoclonal antibodies at 4℃ for 30 min and analyzed using a Coulter Elite flow cytometer (Coulter Electronics Ltd., Hialeah, FL, USA) or a FACSCanto II flow cytometer (BD Pharmingen, San Diego, CA, USA). The monoclonal antibodies used were FITC-conjugated anti-CXCR4, PE-conjugated anti-CXCR4 (clone 12G5; BD Pharmingen, San Diego, CA, USA), and APC-conjugated anti-CXCR7/RDC-1 (clone 11G8; R&D Systems, Minneapolis, MN, USA). To detect cytoplasmic CXCR4 or CXCR7 expression, the cells were permeabilized with a saponin-based reagent (BD Pharmingen, San Diego, CA, USA) and labeled. To detect apoptosis, cells were stained with FITC-conjugated Annexin V and analyzed by flow cytometry.

Kim H.Y., Lee S.Y., Kim D.Y., Moon J.Y., Choi Y.S., Song I.C., Lee H.J., Yun H.J., Kim S, & Jo D.Y. (2015). Expression and functional roles of the chemokine receptor CXCR7 in acute myeloid leukemia cells. Blood research, 50(4), 218-226.

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