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Med24

Manufactured by Fortis Life Sciences
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Sourced in United Kingdom

The MED24 is a versatile laboratory equipment designed for a wide range of scientific applications. It features a compact and durable construction, allowing for efficient use of workspace. The core function of the MED24 is to provide precise temperature control and monitoring capabilities, making it a valuable tool for researchers and scientists working in various fields.

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Lab products found in correlation

β actin, by Merck Group (1 mentions) Vinculin, by Merck Group (1 mentions) Med12, by Cell Signaling Technology (1 mentions) Brca2, by Fortis Life Sciences (1 mentions) β tubulin, by Merck Group (1 mentions) γh2ax, by Merck Group (1 mentions) Rad51, by Abcam (1 mentions)

2 protocols using med24

1

Immunoprecipitation of CDK8 Complex

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WT and MED23/R617Q mutant 293T cells were seeded in 10 cm dishes and cultured in an incubator. At nearly 80% confluence, the cells were harvested and lysed in lysis buffer (1% NP-40, 10% glycerol, 135 mM NaCl, 20 mM Tris, pH 8.0) with protease inhibitors. Lysates were subjected to immunoprecipitation with anti-CDK8 antibody (Santa Cruz, California, USA, #sc-1521) or control IgG at 4°C overnight, followed by washing in lysis buffer, SDS–PAGE electrophoresis and immunoblotting with the indicated antibodies (MED6, Santa Cruz, #sc-9434; Med12, Bethyl, TX, USA, # A300-774A; MED23, Abcam, Cambridge, UK, ab200351; MED24, Bethyl, A300-472A; β-actin, Sigma, MA, USA, A5441).
Yang Y., Li C., Chen Z., Zhang Y., Tian Q., Sun M., Zhang S., Yu M, & Wang G. (2023). An intellectual disability-related MED23 mutation dysregulates gene expression by altering chromatin conformation and enhancer activities. Nucleic Acids Research, 51(5), 2137-2150.
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2

Chromatin Immunoprecipitation Assay Protocol

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CCNC (A301-989A, Bethyl Laboratories), CDK8 (4106S, Cell Signaling Technology), MED12 (4529S, Cell Signaling Technology), MED13 (A301-278A, Bethyl Laboratories), MED24 (A301-472A, Bethyl Laboratories), MED25 (sc-393759, Santa Cruz Biotechnology), BRCA2 (A303-434A, Bethyl Laboratories), Vinculin (#V9131, Sigma), HA (#H3663, Sigma), β-tubulin (#T5168, Sigma), GFP (sc-9996, Santa Cruz Biotechnology), RAD51 (ab63801, Abcam), γH2AX (05-636l, Millipore Sigma).
Tang M., Pei G., Su D., Wang C., Feng X., Srivastava M., Chen Z., Zhao Z, & Chen J. (2021). Genome-wide CRISPR screens reveal cyclin C as synthetic survival target of BRCA2. Nucleic Acids Research, 49(13), 7476-7491.
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