C57BL/6JRj, BALB/c and BALB/c nude mice were purchased from Janvier Labs (France). CD4-dnTGFBR2 (stock number 005551) and C57BL/6J (control for CD4-dnTGFBR2) were purchased from The Jackson Laboratory (USA). All mice were kept in specific-pathogen-free conditions. C57BL/6JRj, BALB/c and BALB/c nude mice were females between 9 and 11 weeks old at the start of the experiment. CD4-dnTGFBR2 and C57BL/6J were 9 weeks old at the start of the experiment.
Balb c nude
Manufactured by Janvier Labs
Sourced in France
BALB/c nude is a strain of laboratory mouse. It is a hairless and immunodeficient mouse model.
Automatically generated - may contain errors
3 protocols using balb c nude
1
Mouse Strains for Immunological Studies
2
Murine Model for Cancer Research
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C57BL/6 mice (8–10 weeks old) were obtained from our mouse facility. BALB/c-nude (6 weeks old) were obtained from Janvier-labs. Housing and all experimental animal procedures were approved by the Institutional Animal Care and Research Advisory Committee of the University of Turin.
3
Small Animal PET Imaging Protocol
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Small animal PET studies were carried out in accordance with the German Animal License Regulations and were approved by the animal care committee of the Regierungspräsidium Freiburg. Imaging was performed on a Focus 120 microPET scanner (Siemens Medical Solutions, USA). For PET imaging studies, nude mice (balb/c nude, Janvier Labs, Le Genest-Saint-Isle, France) (n = 3) were anesthetized with isoflurane (2-4% in air) and placed on the scanner.
Immediately after scan start, animals were injected with 100 µL of 68 Ga-7 (~3.7 MBq) in saline via a catheter into a the tail vein. Data were acquired for 60 min in list mode. Reconstruction was performed using unweighted OSEM2D. Image analysis was performed using AMIDE. Timeactivity curves (TACs) were constructed by manually drawing regions of interest (ROI) over the heart, the liver, the kidneys and the bladder. All ROIs were then copied on each of the frames, and time-activity curves of the ROI mean values were generated.
Immediately after scan start, animals were injected with 100 µL of 68 Ga-7 (~3.7 MBq) in saline via a catheter into a the tail vein. Data were acquired for 60 min in list mode. Reconstruction was performed using unweighted OSEM2D. Image analysis was performed using AMIDE. Timeactivity curves (TACs) were constructed by manually drawing regions of interest (ROI) over the heart, the liver, the kidneys and the bladder. All ROIs were then copied on each of the frames, and time-activity curves of the ROI mean values were generated.
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