The metabolite profiling was performed using a Thermo triple quadrupole tandem MS (TRACE1310 & TSQ8000, Thermo Scientific, United States). The GC separations were carried out using a TG-5SILMS (Thermo Scientific, United States) capillary column (30 m× 0.25 mm× 0.25 µm). Sample volumes of 1 µL were injected with a splitless mode. The front injection temperature was set at 280 °C, the transfer line temperature was set to 280 °C, and the ion source temperature was set at 260 °C, respectively. Helium was the carrier gas with a 1 mL min −1 constant flow rate. The GC temperature program was set isothermally at 50 °C for 1 min, ramped from 50 to 310 °C at a rate of 5 °C min −1, and finally held at 310 °C for 6 min. All mass spectra were acquired in electron impact ionization (EI) mode (70 eV) with a full-scan range of 50-550 m/z at 9 scans per second after a solvent delay of 9 min.
Tg 5silms
Manufactured by Thermo Fisher Scientific
Sourced in United States
The TG-5SILMS is a lab equipment product offered by Thermo Fisher Scientific. It is a thermal gravimetric analyzer that measures changes in the weight of a sample as a function of temperature or time in a controlled atmosphere. The core function of the TG-5SILMS is to provide accurate and reliable thermal analysis data for various materials.
Automatically generated - may contain errors
Lab products found in correlation
Trace 1310, by Thermo Fisher Scientific (1 mentions)
Tsq 8000, by Thermo Fisher Scientific (1 mentions)
Rxi 5ms, by Restek (1 mentions)
Vf 5m, by Agilent Technologies (1 mentions)
Trace 1310 gc, by Thermo Fisher Scientific (1 mentions)
Tracetm1300, by Thermo Fisher Scientific (1 mentions)
Tracefinder 4, by Thermo Fisher Scientific (1 mentions)
Mzcloud, by Thermo Fisher Scientific (1 mentions)
Q exactive gc orbitrap, by Thermo Fisher Scientific (1 mentions)
Compound discoverer 2, by Thermo Fisher Scientific (1 mentions)
Mass frontier 7, by Thermo Fisher Scientific (1 mentions)
Kinetex c18 column, by Phenomenex (1 mentions)
6 protocols using tg 5silms
1
Metabolite Profiling by GC-MS/MS
2
Evaluating GC-Column Impact on Compound Identification
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To test the effect of different GC-columns we measured the Ident-Mixes on the same machine, equipped with three different columns from different vendors. All columns had identical properties with 5% diphenyl, 95% dimethyl polysiloxane coating material, a length of 30 , inner diameter of
and film thickness of
. Tested columns were: TG-5SilMS (Thermo Fisher Scientific, Waltham, MA, USA), Rxi-5ms (Restek, Bad Homburg, Germany) and VF-5ms ((Agilent, Santa Clara, CA, USA)). On the three columns tested, we measured the Ident-Mixes and used Maui-VIA [23 (link)] to identify the substances. Even with considerable retention index shifts, with the help of the Ident-Mix it was still possible to identify the compounds in ca. 1 h per column. In a second run, using newly established RIs, the identifications/verifications took ca. 10–15 .
and film thickness of
. Tested columns were: TG-5SilMS (Thermo Fisher Scientific, Waltham, MA, USA), Rxi-5ms (Restek, Bad Homburg, Germany) and VF-5ms ((Agilent, Santa Clara, CA, USA)). On the three columns tested, we measured the Ident-Mixes and used Maui-VIA [23 (link)] to identify the substances. Even with considerable retention index shifts, with the help of the Ident-Mix it was still possible to identify the compounds in ca. 1 h per column. In a second run, using newly established RIs, the identifications/verifications took ca. 10–15 .
3
GC-MS Analysis of H. Coronarium Extracts
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The chemical compositions of each aromatic extract from H. Coronarium were evaluated by Thermo Scientific™ GC-TRACE 1310, MS-TSQ 9000, column TG-5SILMS 30.0 m × 0.25 mm and film thickness 0.25 μm. The oven was preheated to 70–220 °C to 3 °C/min and treated for 15 min. Injector temperature 250 °C, split flow at 20 mL/min. Helium gas was used as the carrier, set up at 1 mL/min. The headspace was incubated at 100 °C for 3 min. The mass spectrometry ion source and transfer line at 230 °C by scan start mass at 35–550 m/z. The data were acquired and subsequently analyzed using the NIST mass spectral library (Thermo Fisher Scientific, Waltham, MA, USA) as the standard reference database for mass spectral analysis.
4
Characterization of Organic Compounds by GC-MS
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All chemicals were purchased from Alfa Aesar or Sigma-Aldrich.
Gas chromatography-mass spectrometry (GC-MS) analyses were carried out by using a TRACETM 1300 apparatus equipped with an ISQTM QD mass spectrometer detector (Thermo Fisher Scientific). The following chromatographic system was used for analytical experiments: GC-MS (Thermo TG-5SilMS, 20 m, 0.18 µm) with hydrogen as carrier gas [80 °C (0.5 min), followed by linear gradient from 80 to 280 °C (5 min)] at a flow rate of 0.8 mL/min.
Gas chromatography-mass spectrometry (GC-MS) analyses were carried out by using a TRACETM 1300 apparatus equipped with an ISQTM QD mass spectrometer detector (Thermo Fisher Scientific). The following chromatographic system was used for analytical experiments: GC-MS (Thermo TG-5SilMS, 20 m, 0.18 µm) with hydrogen as carrier gas [80 °C (0.5 min), followed by linear gradient from 80 to 280 °C (5 min)] at a flow rate of 0.8 mL/min.
5
Multi-Omics Analysis of Organic Compounds
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GC analyses were carried out on a Thermo Scientific Q Exactive GC Orbitrap. Data were acquired in SIM-SCAN mode from 35-450 AMU. The chromatographic separation was performed on a Thermo Scientific TG-5SILMS (30 m x 250 μm x 0.25 μm) column using Helium carrier as a carrier gas at a constant flow of 1.5 mL/min. Gradient was as follow: initial temperature of 50 °C was held for 5 minutes then ramped to 320 °C at a rate of 15 °C/min and held for 20 minutes, followed by return to 50 °C. Data were analysed with TraceFinder 4.1 and Mass Frontier 7.0 (Thermo Fisher, US) and identification was based on NIST library.
LC analyses were performed on Thermo Scientific Q Exactive LC Orbitrap using full scan mode from 70-1000 AMU. Chromatography was carried out using a Phenomenex Kinetex C18 column (3mm, 100 mm, 2.6 µm). Data were analysed using Mass Frontier 7.0, Compound Discoverer 2.1 (Thermo Fisher) and identification was based on library build with m/z Cloud (Thermo Fisher) and Chemspider.
LC analyses were performed on Thermo Scientific Q Exactive LC Orbitrap using full scan mode from 70-1000 AMU. Chromatography was carried out using a Phenomenex Kinetex C18 column (3mm, 100 mm, 2.6 µm). Data were analysed using Mass Frontier 7.0, Compound Discoverer 2.1 (Thermo Fisher) and identification was based on library build with m/z Cloud (Thermo Fisher) and Chemspider.
6
Volatile Organic Compound Analysis by GC-MS
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Sample preparation was carried out by SPME technique (85 mm Polyacrylate, Fused Silica 24 Ga SPME fiber, adsorption: at 50 8C, for 30 min, desorption: at 200 8C, 0.5 min). GC-MS parameters: column: Thermo Scientific TG-5SILMS 30 m 3 0.25 mm 3 0.25 mm; ion source: 240 8C, ionisation: EI, scan method: FULL, from 5 min, 50-500 mass, scan times: (sec): 0.2, temperature program: 40 8C hold 1 min, 5 8C min À1 to 230 8C hold 5 min. Splitless, Inlet: 200 8C, split flow: 50 mL min À1 , split ratio: 33.3, splitless time: 0.5 min. Septum purge: 5 mL min À1 , vacuum compensation, carrier gas (He), carrier flow: 0.8 mL min À1 .
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