used 650 nm excitation light from an Olympus IX71 inverted confocal
fluorescence microscope. The microscope was fitted with a SPOT Insight
digital camera for fluorescence imaging.
The IX71 is an inverted confocal fluorescence microscope manufactured by Olympus. It is designed for high-resolution imaging of fluorescently labeled samples. The IX71 utilizes a confocal optical system to provide improved optical sectioning and increased signal-to-noise ratio compared to conventional widefield fluorescence microscopy.
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