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3 protocols using siinfekl peptide ova 257 264

1

Immunology Reagents for Cell Culture

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Bafilomycin (B1793), cycloheximide (CHX) (C4859), MG132 (474790), 2-BP (238422), palmostatin B (50–873-80001), and SIINFEKL peptide (OVA 257–264) (S7951) were from Sigma-Aldrich. Cerulenin (10005647) was from Cayman Chemicals. Monensin solution (00–4505-51) was from Thermo Fisher. 2-mercaptoethanol (21985023) was from Gibco. Recombinant human IFNγ (285-IF), human IL-6 (206-IL), and mouse IFNγ (485-MI) were from R & D Systems.
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2

Ferroptosis-associated Compounds Evaluation

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Erastin, RSL3, ML162, ML210, ferrostatin-1, liproxstatin-1, JAK inhibitor I and ruxolitinib were purchased from Cayman Chemical. Doxorubicin (Adriamycin) HCl and gemcitabine were purchased from Selleckchem. Deferoxamine mesylate salt, L-Glutathione reduced, sulfasalazine, L-Buthionine-sulfoximine, 2-Mercaptoethanol, and SIINFEKL peptide (OVA 257–264) were purchased from Sigma-Aldrich. Recombinant human IFNγ (285-IF) and mouse IFNγ (485-MI) were purchased from R&D. BODIPY 581/591 C11, anti-IFNγ (XMG1.2) and anti-TNFα (MP6-XT22) blocking antibodies were purchased from Thermo Fisher Scientific. Liperfluo was purchased from Dojindo Molecular Technologies. Cyst(e)inase was obtained from the laboratory of Everett Stone and George Georgiou (University of Texas at Austin, TX, USA).
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3

Ferroptosis-associated Compounds Evaluation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Erastin, RSL3, ML162, ML210, ferrostatin-1, liproxstatin-1, JAK inhibitor I and ruxolitinib were purchased from Cayman Chemical. Doxorubicin (Adriamycin) HCl and gemcitabine were purchased from Selleckchem. Deferoxamine mesylate salt, L-Glutathione reduced, sulfasalazine, L-Buthionine-sulfoximine, 2-Mercaptoethanol, and SIINFEKL peptide (OVA 257–264) were purchased from Sigma-Aldrich. Recombinant human IFNγ (285-IF) and mouse IFNγ (485-MI) were purchased from R&D. BODIPY 581/591 C11, anti-IFNγ (XMG1.2) and anti-TNFα (MP6-XT22) blocking antibodies were purchased from Thermo Fisher Scientific. Liperfluo was purchased from Dojindo Molecular Technologies. Cyst(e)inase was obtained from the laboratory of Everett Stone and George Georgiou (University of Texas at Austin, TX, USA).
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