Blebbistatin

Manufactured by Beyotime

Sourced in China

Blebbistatin is a small molecule compound that functions as a selective inhibitor of non-muscle myosin II. It acts by binding to the myosin II motor domain and blocking its ATPase activity, thereby inhibiting the contractile function of non-muscle myosin II.

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Lab products found in correlation

Ns 398, by Beyotime (1 mentions) Y27632, by Beyotime (1 mentions)

2 protocols using blebbistatin

Modulating Spine Follicle Development

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ATPase inhibitor (Blebbistatin, 1mM, Beyotime) and COX2 inhibitor (NS-398, 50 µM, Beyotime) were injected into the bottom of the spine follicle at the early growth phase using a microinjector, with DMSO as a control. Spine follicles were injected for two consecutive days and collected on the third and ninth days to observe their phenotypes using HE and immunofluorescence staining.

Zhang M., Wang M., Jiang J., Liu W., Zhou S., Wang D., Wang M., Zhao Z., Xu Z., Wu W., Lin X., Zhang J., Xu W., Tang Q., Zhan R., Liu W., Yang L., Zhou X., Zhou W, & Lei M. (2023). COX2-ATP Synthase Regulates Spine Follicle Size in Hedgehogs. International Journal of Biological Sciences, 19(15), 4763-4777.

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Pharmacological Inhibition of Cell Mechanics

Cited 2 times

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For pharmacological inhibition studies, the inhibitors were added to the induction medium following 1 day of culture. The concentrations used for each inhibitor were 10 μM for Y-27632 (Beyotime, China) and 5 μM for Blebbistatin (Beyotime, China). These concentrations matched those used in similar studies (74 (link), 75 (link)).

Huang D., Li Y., Ma Z., Lin H., Zhu X., Xiao Y, & Zhang X. (2023). Collagen hydrogel viscoelasticity regulates MSC chondrogenesis in a ROCK-dependent manner. Science Advances, 9(6), eade9497.

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