Cd11b bv785 clone icrf44

Manufactured by BioLegend

Sourced in United States

CD11b BV785 (clone ICRF44) is a fluorescently-labeled monoclonal antibody that binds to the CD11b protein. CD11b is an integrin that is expressed on the surface of various immune cells, including monocytes, macrophages, and neutrophils. This antibody can be used to identify and study these cell populations in flow cytometry applications.

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Lab products found in correlation

Cd3 alexa700 clone sp34 2, by BD (1 mentions) Live dead fixable aqua dead cell dye, by Thermo Fisher Scientific (1 mentions) Facsaria 2 flow cytometer, by BD (1 mentions) Facsdiva software, by BD (1 mentions) Pharm lyse lysing buffer, by BD (1 mentions) Hla dr pe clone immu 357, by Beckman Coulter (1 mentions) Cd56 apc clone n901, by Beckman Coulter (1 mentions) Cytoflex flow cytometer, by Beckman Coulter (1 mentions)

2 protocols using cd11b bv785 clone icrf44

Polychromatic Immunophenotyping of Rhesus Macaque Brain

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Polychromatic cell sorting was performed on stained mononuclear cells isolated from fresh rhesus macaque brain tissue utilizing a FACSAria II flow cytometer (BD Biosciences, Franklin Lakes, NJ) equipped with the FACSDiva software (BD Biosciences). Cells were first stained with a LIVE/DEAD fixable aqua dead cell dye (Life Technologies, Carlsbad, CA). For live-cell sorting, the following panel of monoclonal antibodies (MAbs) was used: CD14 fluorescein isothiocyanate (FITC; clone M5E2), BD CD123 phycoerythrin (PE; clone 6H6; Pharmingen), CD28 ECD (clone CD28.2; BioLegend), CD95 PECy5 (clone DX2; Beckman Coulter), CD11c BV605 (clone 3.9; BioLegend), HLA-DR APC-H7 (clone L243; BioLegend), CD4 BV650 (clone OKT4; BD), CD11b BV785 (clone ICRF44; BioLegend), CD45 V450 (clone D058-1283; BioLegend), CD206 APC (clone 19.2; BD Horizon), CD3 Alexa700 (clone SP34-2; BD Pharmingen), and CD20 PE-Cy7 (clone L27; BD Pharmingen). The results were analyzed using the FlowJo software v9.9 (TreeStar, Ashland, OR). A threshold cutoff of 200 cells of the parent population was used for all cell subset analyses.

Lee C.A., Beasley E., Sundar K., Smelkinson M., Vinton C., Deleage C., Matsuda K., Wu F., Estes J.D., Lafont B.A., Brenchley J.M, & Hirsch V.M. (2020). Simian Immunodeficiency Virus-Infected Memory CD4+ T Cells Infiltrate to the Site of Infected Macrophages in the Neuroparenchyma of a Chronic Macaque Model of Neurological Complications of AIDS. mBio, 11(2), e00602-20.

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Characterizing Monocyte Subsets and Platelet Complexes

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Monocyte subpopulations, platelet complexes and expression markers were identified with flow cytometry. Using the lysis‐no‐wash strategy (BD Pharm Lyse lysing buffer, Becton Dickinson, Franklin Lakes, NJ, USA), 50 μL EDTA blood was stained by monoclonal antibodies (CD45 Chrome Orange clone J33 Beckman Coulter, HLA‐DR PE clone immu‐357 Beckman Coulter, CD14 PC7 clone 61D3 Bioscience, CD16 FITC clone CB16 eBioscience, CD3 APC‐750 clone UCTH1 Beckman Coulter, CD56 APC clone N901 Beckman Coulter, CD192 BV421 clone 48607 Becton&Dickinson, CD11b BV785 clone ICRF44 Biolegend, CD41 PC5.5 clone Hip8 Biolegend) and measured with CytoFLEX flow cytometer (Beckman Coulter, Brea, CA, USA). The gating strategy applied is shown in Figure S1, gates were set with the fluorescence minus one method.24, 25 Data were analyzed with Kaluza 3.1 software (Beckman Coulter). Characterization of monocytes subsets is according to current recommendations.24, 25

Noz M.P., Hartman Y.A., Hopman M.T., Willems P.H., Tack C.J., Joosten L.A., Netea M.G., Thijssen D.H, & Riksen N.P. (2019). Sixteen‐Week Physical Activity Intervention in Subjects With Increased Cardiometabolic Risk Shifts Innate Immune Function Towards a Less Proinflammatory State. Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, 8(21), e013764.

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