Fine chemicals such as streptozotocin, metformin hydrochloride, and nicotinamide were procured from Cayman Chemical, USA. ELISA kits for estimation of insulin, acetylcholine, acetylcholinesterase were procured from Cloud-Clone Corp., USA. Other chemicals and solvents were purchased locally with analytical grades.
Nicotinamide
Manufactured by Cayman Chemical
Sourced in United States
Nicotinamide is a chemical compound that functions as a precursor to the coenzyme nicotinamide adenine dinucleotide (NAD). NAD plays a crucial role in cellular metabolism, serving as an electron carrier in various redox reactions. Nicotinamide is commonly used in laboratory settings for research purposes.
Automatically generated - may contain errors
Lab products found in correlation
Sb216763, by Cayman Chemical (2 mentions)
Aminopterin, by Cayman Chemical (2 mentions)
Vitamin e α tocopherol acetate, by Cayman Chemical (2 mentions)
Ci994, by Cayman Chemical (2 mentions)
Sb 415286, by Cayman Chemical (2 mentions)
Chir99021, by Cayman Chemical (2 mentions)
Streptozotocin (stz), by Cayman Chemical (1 mentions)
Metformin hydrochloride, by Cayman Chemical (1 mentions)
Lithium chloride (licl), by Merck Group (1 mentions)
Trichostatin a, by Cayman Chemical (1 mentions)
Resveratrol, by Cayman Chemical (1 mentions)
Trichostatin a tsa, by Cayman Chemical (1 mentions)
3 protocols using nicotinamide
1
Pharmacological Agents in Biochemical Assays
2
SOSR-CO Pharmacological Modulation
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Immediately after plating in 96-well plates, the SOSR-COs were treated with various drugs for 48 h. Along with VPA (Cayman Chemical), the SOSR-COs were treated with different HDAC inhibitors, GSK3β inhibitors, folic acid (FA) inhibition or supplementation, and antioxidants. We tested HDAC inhibitors that target different classes: Trichostatin A (Cayman Chemical), Nicotinamide (Cayman Chemical), and CI-994 (Cayman Chemical). The GSK3β inhibitors included CHIR99021 (Cayman Chemical), BIO (Cayman Chemical), SB-216763 (Cayman Chemical), SB-415286 (Cayman Chemical), and lithium chloride (Sigma-Aldrich, St. Louis, MO, USA). FA (Cayman Chemical) was supplemented, and the FA pathway was inhibited by aminopterin (Cayman Chemical). The antioxidant was vitamin E (±-α-Tocopherol Acetate; Cayman Chemical). Appropriate amounts of each treatment were mixed with 3N media without vitamin A, plus the 4 inhibitors. A total of 100 μL of media mixed with the treatment was added per well.
3
Modulation of SOSR cell fate
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Immediately after plating in 96-well plates, SOSRS were treated with various drugs for 48 hours. Along with VPA (Cayman Chemical, 13033), SOSRS were treated with different HDAC inhibitors, GSK3β inhibitors, folic acid (FA) inhibition or supplementation, and antioxidants. We tested HDAC inhibitors that target different classes: Trichostatin A (TSA; Cayman Chemical, 89730), Nicotinamide (Cayman Chemical, 11127), CI-994 (Cayman Chemical, 12084). GSK3β inhibitors included CHIR99021 (Cayman Chemical, 13122), Bio (Cayman Chemical, 13123), SB-216763 (Cayman Chemical, 10010246), SB-415286 (Cayman Chemical, 10010247), and lithium chloride. FA (Cayman Chemical, 20515) was supplemented, and the FA pathway was inhibited by Aminopterin (Cayman Chemical, 21802). Antioxidants included Vitamin E (±-α-Tocopherol Acetate; Cayman Chemical, 28399), and Resveratrol (trans-Resveratrol [RV]; Cayman Chemical, 70675). Appropriate amounts of each treatment were mixed with 3N media without vitamin A plus the 4 inhibitors. 100μL of media mixed with the treatment was added per well. SOSRS were treated for 48 hours before being fixed.
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