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3 protocols using 2 o dibutyryladenosine 3 5 cyclic monophosphate dbcamp

1

Modulation of cAMP and Ca2+ Signaling

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A cAMP analog N6, 2′-O-dibutyryladenosine 3′, 5′-cyclic monophosphate (db-cAMP) and various Ca2+ modulators nifedipine, verapamil, dantrolene, alamethicin, and ionomycin were purchased from Sigma-Aldrich (St. Louis, MO). Forskolin, an activator of AC, was obtained from Applichem (Darmstadt, Germany). O,O'-Bis (2-aminophenyl)ethyleneglycol-N,N,N',N'-tetraacetic acid, tetraacetoxymethyl ester; BAPTA)was commercially provided by Dojindo (Kumamoto, Japan)
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2

DMBA, dbcAMP, and Sodium Orthovandate Protocol

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7,12- Dimethylbenz(a)anthracene (DMBA) (≥95% purity), N6, 2’-O-dibutyryladenosine 3:5-cyclic monophosphate (dbcAMP) and Sodium orthovandate (Na3VO4) were purchased from Sigma Aldrich Chemical Co. (St. Louis, MO). 12-O-tetradecanoyl-phorbol-13-acetate (TPA) was obtained from LC laboratories (Woburn, MA).
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3

Immune Response Modulation Protocol

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7,12-dimethylbenz(a)anthracene (DMBA) (≥ 95% purity), N6, 2’-O-dibutyryladenosine 3:5-cyclic monophosphate (dbcAMP) and Sodium orthovandate (Na3VO4) were purchased from Sigma Aldrich Chemical Co. (St. Louis, MO). 12-O-tetradecanoyl-phorbol-13-acetate (TPA) was obtained from LC laboratories (Woburn, MA). Rat anti-mouse IL-12Rβ2 and IL-23R were purchased from R&D; Rabbit anti-mouse VEGF, TRP2, Mouse anti-human S100, Rat anti-mouse vimentin were obtained from Santa Cruz Biotechnology, Inc. Rat anti-mouse pERK was from BD biosciences. CD4-PE, CD4-FITC, FOXP3-PE, FOXP3-v450, IA/IE-FITC, IL-17-Percp-Cy5.5, IL-10-PE, CD45.2-Percp-Cy5.5, CD45.2-FITC were obtained from eBiosciences. IFNγ-PE-Cy7, CD8-Alexa-647, and CD8-PE were obtained from BD-pharmingen.
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