We evaluated the impact of CGRP signalling on the outcome of bacterial meningitis by treating mice with CGRP injection or with the CGRP antagonist BIBN4096 (Tocris). For these experiments, mice were treated with CGRP (0.1 mg kg−1), BIBN4096 (0.3 mg kg−1) or vehicle by intraperitoneal injection. Treatments were performed 2 h before induction of bacterial meningitis as described in the section ‘Haematogenous bacterial meningitis ’ and again 24 h later. Additionally, the role of IL-10 on the effects of CGRP was determined by treating mice with vehicle or CGRP and either isotype control antibody or neutralizing anti-IL-10 antibody (Bio-X-Cell, 200 μg in 100 μl, intraperitoneally, daily). Treatment doses were selected on the basis of previous publications using these compounds22 (link),57 (link).
Bibn4096
Manufactured by Bio-Techne
Sourced in United Kingdom
BIBN4096 is a laboratory instrument designed for the detection and analysis of biomolecules. It functions as a high-performance analytical tool for researchers and scientists working in the life sciences and biotechnology fields.
Automatically generated - may contain errors
Lab products found in correlation
Capsaicin, by Merck Group (2 mentions)
Indomethacin, by Merck Group (2 mentions)
Biie0246, by Bio-Techne (2 mentions)
Bibo3304, by Bio-Techne (2 mentions)
Lacl3, by Merck Group (1 mentions)
Endothelial cell growth supplement (ecgs), by Merck Group (1 mentions)
Probenecid, by Merck Group (1 mentions)
Ethidium bromide, by Merck Group (1 mentions)
10panx, by GenScript (1 mentions)
Cgrp8 37, by GenScript (1 mentions)
Lucifer yellow, by Thermo Fisher Scientific (1 mentions)
Ppads, by Bio-Techne (1 mentions)
Fitc dextran, by Merck Group (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Collagenase type 1, by Worthington (1 mentions)
Tetrodotoxin ttx, by Abcam (1 mentions)
Sodium propionate, by Merck Group (1 mentions)
αcgrp, by Bachem (1 mentions)
Cgrp8 37, by Bachem (1 mentions)
Ezrmgip 55k, by Merck Group (1 mentions)
11 protocols using bibn4096
1
Role of CGRP in Bacterial Meningitis
2
Systemic BoNT/A Pretreatment Modulates CFT073 Infection
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
BoNT/A (0.2 U, Lanzhou biology) was systemically administrated by intraperitoneal injection 24 hours before infection with CFT073, and BIBN4096 treatment (Tocris #4561) was systemically administrated by intraperitoneal injection 2 hours before infection.
3
Pharmacological Modulation of Cellular Signaling
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Capsaicin, L-NA, indomethacin, MOPS, ethidium bromide, ECGS, FITC-dextran (3000 Da), BSA, PE, ACh, LaCl3 and probenecid were purchased from Sigma-Aldrich (St. Louis, MO, USA). PPADS and BIBN4096 were obtained from Tocris Bioscience (Ellisville, MO, USA), SNAP and TEMPOL from Calbiochem (La Jolla, CA, USA), Lucifer Yellow (LY) from Molecular Probes (Eugene, OR, USA), CGRP (alpha isoform, rat) from Bachem (Torrance, CA, USA) and collagenase type I from Worthington (Lakewood, NJ, USA). CGRP8–37, 10panx, 37,43GAP27 and 40GAP27 were synthesized by Genscript (Israel). Capsaicin was dissolved in ethanol; indomethacin, BIBN4096 and SNAP in dimethyl sulfoxide (DMSO) and probenecid in 0.5 M NaOH. Then, these drugs were diluted in the buffer solution to the final working concentration. Application of the vehicle of these drugs (ethanol, DMSO or NaOH) did not have effect per se (data not shown).
4
CGRP Receptor Antagonist in Mice Challenge
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Mice were injected with CGRP receptor antagonist BIBN4096 (cat. 4096, Tocris, Bristol, UK) intraperitoneally (30 mg/kg) one hour after the infectious challenge [19 (link)]. The reagent was prepared on the day of the challenge per manufacturer’s instructions. Control mice were injected with the vehicle (20% DMSO, 1.5% Tween-80 in PBS).
5
Pharmacological Compounds and Reagents
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
BIBO3304, BIIE0246, BIBN4096, and phloridzin were purchased from Tocris (Bristol, UK). Stock solutions of BIBO3304, BIIE0246, and BIBN4096 were dissolved in 10% dimethyl sulfoxide (DMSO, at 1 m mol L−1) and were stored at −20°C. The FFA2 agonist, PA was purchased from Calbiochem (Watford, UK), peptides were from Cambridge Bioscience (Cambridge, UK) and stock aliquots were stored at −20°C, undergoing one freeze‐thaw cycle only. Tetrodotoxin (TTX) was purchased from Abcam (Cambridge) while all other agents, including sodium propionate, were from Sigma (Poole, UK).
6
CGRP Receptor Antagonists Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The chemicals and drugs used in this study were as follows: α-CGRP and CGRP8–37 were obtained from BACHEM AG (Bubendorf, Switzerland). BIBN 4096 were purchased from Tocris Cookson (Bristol, UK) and PTX was bought from Sigma-Aldrich (St Louis, MO, USA). All the other drugs were purchased from HelloBio (Princeton, NJ, USA) except NB001, which was provided by NeoBrain Pharmac Inc. (Canada). Drugs were prepared as stock solutions for frozen aliquots at − 20 °C. All these drugs were diluted from the stock solution to the final desired concentration in the ACSF before being applied to the perfusion solution.
7
Pharmacological Evaluation of Gut Hormone Modulators
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
PEG200, HCl, Pefabloc, and acetaminophen were purchased from Sigma Aldrich (Cat. No. P3015, 1.00317, 76,307, and A7085; Soborg, Denmark). BIBO3304 (Y1 antagonist), BIIE0246 (Y2 antagonists), and BIBN4096 (CGRP antagonist) were purchased from Tocris (Cat. No. 2412, 1700 and 4561; Bristol, UK). Somatostatin receptor antagonist was purchased from Bachem (iSST, Cat. No. BIM-23627; Bubendorf, Switzerland). Exendin 4 (Ex4) and Exendin (9–39) (Ex(9–39)) were both purchased from Anaspec (Cat. No. ANA24463 and ANA24467; Cambridge Bioscience, Cambridge, UK). Obestatin was kindly provided by Annette Beck-Sickinger and synthesized as previously described [12 (link)]. ELISAs for GLP-1 and insulin were obtained from MesoScale (Cat. No. L4503PA and K152BZC; MesoScale Diagnostics, Maryland, USA). GIP and total ghrelin were acquired from Merck/Millipore (Cat. No. EZRMGIP-55K and EZRGRT-91K; Merck/Millipore Darmstadt, Germany). PYY ELISA was purchased from Crystal Chem (Cat. No. 81501; Zaandam, Netherlands). An acetaminophen kit was obtained from Sekisui Diagnostics (Cat. No. 506–10; Sekisui, Lexington, MA, USA). Cpd1324 was synthesized by InterBioScreen Ltd. as previously described [10 (link)] and formulated with a vehicle containing 3.2 μM (−5.5 M) of zinc ions for all of the in vivo experiments.
8
Pharmacological Agents for Experimental Research
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Evans Blue dye and GSK1016790A were purchased from Sigma-Aldrich (St. Louis, MO); 5-HT, HC067047, SR140333, GF 109203X (GFX), and BIBN 4096 (Olcegepant) were purchased from Tocris Bioscience (Bristol, UK); YM 26734 was from Cayman Chemical; WAY-100635 Maleate, GR 55562 dihydrochloride, GR113808 and SB 269970 hydrochloride were purchased from Abcam Australia (Melbourne, VIC Australia). Ketanserin and RS-127445 were purchased from Selleck Chemicals (Houston, TX. USA); Evans Blue was dissolved in sterile 0.9% saline. All drugs administered to mice were prepared on the day of experimentation in sterile 1% dimethyl sulfoxide (DMSO) in 0.9% saline.
9
Pharmacological Agents for Biochemical Research
10
Trpv1+ Neuron Ablation via Systemic and Intrathecal RTX
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For chemical ablation of Trpv1+ neurons with systemically administered resiniferatoxin (RTX, Sigma Aldrich), 4-week-old C57BL/6 mice were injected subcutaneously in the flank with escalating doses of RTX (30, 70, 100 µg/kg on consecutive days) or vehicle (2% DMSO/0.15% Tween-80 in PBS). For chemical ablation of Trpv1+ neurons with intrathecally administered RTX, 4-week-old C57BL/6 mice were injected intrathecally near the iliac crest with two daily doses of RTX (25 ng) or vehicle (0.25% DMSO/0.02% Tween-80 in PBS). BIBN 4096 (Tocris; 50 pmol in 10 µL) and its vehicle (0.05% DMSO in saline), Spantide I (Tocris; 5 nmol in 10 µL) and its vehicle (water) were injected subcutaneously into the ipsilateral footpad using a 100 µL Hamilton syringe and 32-gauge needle under isoflurane anesthesia. The antagonists and vehicle controls were administered 15 min prior to ET.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!