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Bio plex pro human cytokine 17 plex panel

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The Bio-Plex Pro Human Cytokine 17-Plex Panel is a multiplex assay kit designed to detect and quantify 17 different human cytokines simultaneously in a single sample. The panel allows for the measurement of multiple analytes, including IL-2, IL-4, IL-6, and TNF-α, using flow cytometry technology.

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Lab products found in correlation

Bio plex manager, by Bio-Rad (2 mentions) Bio plex 200 system, by Bio-Rad (1 mentions) Luminex 200 system, by Bio-Rad (1 mentions) Bio plex pro human cytokine ip 10, by Bio-Rad (1 mentions) Bio plex pro human cytokine ifn a2, by Bio-Rad (1 mentions) Ficoll, by Cytiva (1 mentions) Limulus amebocyte lysate assay, by Lonza (1 mentions) Carboxyfluorescein succinimidyl ester (cfse), by Tree Star (1 mentions) Anti biotin macsibead particles, by Miltenyi Biotec (1 mentions) Carboxyfluorescein succinimidyl ester (cfse), by Thermo Fisher Scientific (1 mentions) Carboxyfluorescein succinimidyl ester (cfse), by Miltenyi Biotec (1 mentions) Bio plex 200, by Bio-Rad (1 mentions)

Spelling variants of this product

Bio-Plex (286 citations) Bio-Plex Human Cytokine 17-Plex Panel (4 citations) Bio-Plex Pro human cytokine 17-plex (2 citations) Human 17-Plex Panel (2 citations) Human Bio-Plex Pro (2 citations)

7 protocols using bio plex pro human cytokine 17 plex panel

1

Multiplex Cytokine Profiling in Serum

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A multiplex assay for quantitative determination of inflammatory mediators was applied to assess the concentrations of selected cytokines in serum. The analysis was performed using the Bio-Plex Pro Human Cytokine 17-Plex panel (Bio-Rad Laboratories GmbH). The following determinants were simultaneously detected: IL-1β, IL-2, IL-4, IL-5, IL6, IL-7, IL-8, IL-10, IL-12, IL-13, IL-17, G-CSF, GM-CSF, IFN-γ, MCP-1, MIP-1β and TNF-α. Multiplexing was performed according to the manufacturer’s instruction manual and analyzed on a Bio-Rad Bio-Plex 200 system. Values were calculated by the Bio-Plex™ software (Bio-Plex Manager, version 6.1, Bio-Rad). All serum samples were measured in triplicates.
Schimmack S., Yang Y., Felix K., Herbst M., Li Y., Schenk M., Bergmann F., Hackert T, & Strobel O. (2019). C-reactive protein (CRP) promotes malignant properties in pancreatic neuroendocrine neoplasms. Endocrine Connections, 8(7), 1007-1019.
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2

Multiplex Cytokine Profiling of Activated pDCs

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Initial screening of cytokine concentrations in the supernatant of the stimulated pDCs was performed using Bio-Plex Pro Human Cytokine 17-Plex Panel (Bio-Rad), complemented by a Bio-Plex Pro Human Cytokine IP-10 (Bio-Rad) and Bio-Plex Pro Human Cytokine IFN-a2 (Bio-Rad) resulting in a total of 19 cytokine targets (IP-10, MIP-1β, TNF-α, IFN-α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12, IL-13, IL-17, G-CSF, GM-CSF, IFN-γ and MCP-1). Consecutive analyses were performed using a combination of Bio-Plex Pro Human Cytokine IP-10, Bio-Plex Pro Human Cytokine MIP-1α, Bio-Plex Pro Human Cytokine TNF-α and Bio-Plex Pro Human Cytokine IFN-a2 sets (Bio-Rad).
Multiplex assay was performed on a Luminex 200 system (Bio-Rad) in accordance with the manufacturer’s instructions. Standards were analysed in duplicates and each sample in triplicates. Washing was performed between each step using a HydroFlex microplate equipped with a magnetic plate carrier (Tecan). Analysis of the data was performed using Bio-Plex Manager (Bio-Rad).
Bucher K., Rodríguez-Bocanegra E., Wissinger B., Strasser T., Clark S.J., Birkenfeld A.L., Siegel-Axel D, & Fischer M.D. (2023). Extra-viral DNA in adeno-associated viral vector preparations induces TLR9-dependent innate immune responses in human plasmacytoid dendritic cells. Scientific Reports, 13, 1890.
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3

Reducing Endotoxin for T Cell Assays

Cited 2 times
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To reduce endotoxin concentrations in proteins used for T cell assays, each of the Ags was subjected to affinity chromatography using a polymyxin matrix (Bio-Rad, Hercules, California). The endotoxin contents in the protein solutions were determined by the Limulus Amebocyte Lysate assay (Lonza, Basel, Switzerland) and were found to be in the range of 12.4–54 ng/mg of protein. Heparinized venous blood samples were collected from six patients with HDM allergy. PBMCs were isolated by Ficoll (Amersham Bioscience, Uppsala, Sweden) and incubated in control medium or 1.25 μM recombinant Der p 23, PreS-2XP4P5, PreS, peptide 4 (P4), or peptide 5 (P5) for 6 d (37°C). Proliferation assays were performed as previously described, and results were expressed as stimulation index (33 (link)). The cultured cell supernatants from the lymphocyte proliferation assays were used to determine the concentrations of cytokines with the Bio-Plex Pro Human Cytokine 17-Plex Panel (Bio-Rad, Hercules, CA), according to the manufacturer’s instructions, as described (25 (link)).
Banerjee S., Weber M., Blatt K., Swoboda I., Focke-Tejkl M., Valent P., Valenta R, & Vrtala S. (2014). Conversion of Der p 23, a New Major House Dust Mite Allergen, into a Hypoallergenic Vaccine. Journal of immunology (Baltimore, Md. : 1950), 192(10), 4867-4875.
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4

Plasma Cytokine Profiling in Weight Loss

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Levels of cytokines were assessed in plasma of patients enrolled in the study using the Bio-Plex Pro human cytokine 17-plex panel (M5000031YV, Bio-Rad) according to the manufacturer’s protocol [21 (link)]. The secretome profile was assessed at baseline (T0) and after diet/weight loss (T1) and a delta (T1–T0) of plasma cytokine level has been also calculated.
Montefusco L., D’Addio F., Loretelli C., Ben Nasr M., Garziano M., Rossi A., Pastore I., Plebani L., Lunati M.E., Bolla A.M., Porta M.D., Piuri G., Rocchio F., Abdelsalam A., Assi E., Barichella M., Maestroni A., Usuelli V., Loreggian L., Muzio F., Zuccotti G.V., Cazzola R, & Fiorina P. (2021). Anti-inflammatory effects of diet and caloric restriction in metabolic syndrome. Journal of Endocrinological Investigation, 44(11), 2407-2415.
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5

Th Cell Suppression by Regulatory T Cells

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Th cells and Tregs were sorted from PBMCs using a human CD4+CD25+Regulatory T Cell Isolation Kit (MACS, Miltenyi Biotec). Th cells were labeled with carboxyfluorescein succinimidyl ester (CFSE, Molecular Probes) according to manufacturer’s protocol. CFSE-labeled Th cells were stimulated with Anti-Biotin MACSiBead™ Particles (MACS, Miltenyi Biotec) and co-cultured with unlabeled Tregs. Th cell division was quantified based on serial halving of CFSE intensity, using algorithms provided by FlowJo software (Treestar).
Cytokine measurements in supernatants of co-cultures were performed using the Bio-Plex Pro Human Cytokine 17-plex Panel (Biorad), run on a Luminex 100 System (Luminex Corporation), according to manufacturer’s protocol.
Broos C.E., van Nimwegen M., Kleinjan A., ten Berge B., Muskens F., in ’t Veen J.C., Annema J.T., Lambrecht B.N., Hoogsteden H.C., Hendriks R.W., Kool M, & van den Blink B. (2015). Impaired survival of regulatory T cells in pulmonary sarcoidosis. Respiratory Research, 16(1), 108.
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6

Plasma Cytokine Profiling Protocol

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Blood samples were drawn into EDTA coated collection tubes and transported to our laboratory at room temperature, which is approximately a five-minute walk. Blood was immediately centrifuged at 8000rpm for 10min at 4°C. Plasma supernatant was collected and frozen in the −80°C freezer in order to measure pro- and anti-inflammatory cytokine levels in circulation. At time of measurement, plasma was thawed on ice and cytokine levels in all plasma samples were determined by magnetic bead suspension array using the Bio-Plex Pro Human Cytokine 17-plex panel (Bio-Rad Laboratories, Hercules, CA, USA). Following manufacturer protocol instructions, 100 uL/ well of undiluted plasma was used and run in duplicate. The panel included the cytokines: IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12(p70), IL-13, IL-17, G-CSF, GM-CSF, IFN-γ, MCP-1, MIP-1β, TNF-α.
Cannon A.R., Anderson L.J., Galicia K., Murray M.G., Kamran A.S., Li X., Gonzalez R.P, & Choudhry M.A. (2023). Traumatic Brain Injury Induced Inflammation and GI Motility Dysfunction. Shock (Augusta, Ga.), 59(4), 621-626.
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7

Multiplex Cytokine Profiling in Serum

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Cytokines in serum samples were measured by a fluorescent suspension array system Bio‐Plex 200 (Bio‐Rad) using a Bio‐Plex Pro Human Cytokine 17‐Plex Panel (#M5000031YV) according to the manufacturer's protocol. Bio‐Plex 200 system was validated by a Validation Kit (#171203001) within 1 week of assays and calibrated by a Calibration Kit (#171203060) on each assay day. The quantifications of IL‐2, IL‐4, IL‐5, IL‐6, IL‐10, IL‐13, IFN‐γ, and TNF‐α were included in this study.
Liu Y., Li W., Qian J., Wu M., Du H., Xu L., Liu S., Yi J, & He G. (2020). Serum phytosterols associate with T helper 1 cytokine concentration in pregnant women. Food Science & Nutrition, 8(7), 3893-3899.
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