Pe cy7 tagged cd45

Manufactured by BD

Sourced in United States

PE/Cy7-tagged CD45 is a flow cytometry reagent that utilizes a combination of the fluorescent dyes PE and Cy7 to label the CD45 protein. CD45 is a transmembrane protein tyrosine phosphatase expressed on the surface of most hematopoietic cells. This reagent can be used to identify and enumerate CD45-positive cells in a sample.

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Lab products found in correlation

Alexa fluor 488 tagged integrin β1, by Santa Cruz Biotechnology (2 mentions) Alexa fluor 700 tagged cd11b, by BD (2 mentions) Anti akt2, by Cell Signaling Technology (2 mentions) Integrin α4, by Cell Signaling Technology (1 mentions) Anti icam 1, by Cell Signaling Technology (1 mentions) Anti akt, by Cell Signaling Technology (1 mentions) Vcam 1, by Abcam (1 mentions) Anti neutrophil elastase, by Abcam (1 mentions) Anti stat1, by Cell Signaling Technology (1 mentions) Anti dab2, by Cell Signaling Technology (1 mentions) Anti myeloperoxidase mpo, by R&D Systems (1 mentions) Anti human cd34 antibody, by BD (1 mentions)

Close spelling variants of this product

CD45-PE (130 citations) CD45-PE-Cy7 (58 citations)

2 protocols using pe cy7 tagged cd45

Multicolor Flow Cytometry of Leukocyte Subsets

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Antibodies for flow cytometry which were purchased from BD Bioscience, Franklin Lakes, NJ, USA are PE/Cy7-tagged CD45 (Cat# 103114), BV421-tagged Ly6C (Cat# 128016), APC-tagged Ly6G (Cat# 560599), Alexa fluor 700-tagged CD11b (Cat# 557960). Other antibodies used are PerCP-Cy5.5-tagged CX3CR1 (Biolegend, San Diego, CA, USA; Cat# 149009), FITC-tagged CD14 (Thermo Fisher, Waltham, MA, USA; Cat# 11-0141-81), anti-AKT2 (Cell Signaling Technologies, Danvers, MA, USA; Cat# 2964S), Alexa fluor 488-tagged integrin β1 (Santa Cruz Biotechnology, Dallas, TX, USA; Cat# sc-374429 AF488), integrin α4 (Cell Signaling Technologies, USA; 8440S), and Actin (Cell Signaling Technologies, USA; Cat# 4970S).

Boyce M., Xin Y., Chowdhury O., Shang P., Liu H., Koontz V., Strizhakova A., Nemani M., Hose S., Zigler JS J.r., Campbell M., Sinha D., Handa J.T., Kaarniranta K., Qian J, & Ghosh S. (2022). Microglia–Neutrophil Interactions Drive Dry AMD-like Pathology in a Mouse Model. Cells, 11(22), 3535.

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Multiparameter Flow Cytometry Panel

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PE/Cy7-tagged CD45 (Cat# 103114), APC-tagged Ly6C (Cat# 128016), FITC-tagged CD66b (Cat# 555724), V450-tagged Ly6G (Cat# 560603), Alexa fluor 700-tagged CD11b (Cat# 557960), anti-human PE/Cy7-tagged CD45 (Cat# 560178), and Anti human CD34 antibody (Cat# 343602) were purchased from BD Biosciences, USA and anti-human PE-tagged IL-28AR antibody (Cat# 337804) was purchased from Biolegend, USA. Anti-Neutrophil Elastase (Cat# ab68672), anti-GRO alpha (CXCL1) (Cat# ab86436), Anti-STAT1 (phosphor S727) (Cat# ab109461), anti-IL28 receptor alpha or IL28R1 (Cat# ab224395), anti-Histone H3 citrunillated (Cat# ab219407), VCAM1 (Cat# ab134047), CD34 (Cat# 8158) and IL28 + IL29 (Cat# ab191426) antibodies were purchased from Abcam, USA. Anti-ICAM-1 (Cat# SC-107), Anti-STAT1 (Cat# 9172T), anti-AKT (Cat# 4685S), anti-AKT2 (Cat# 2964S) and anti-DAB2 (Cat# 12906S) were purchased from Cell Signaling Technologies, USA. Other antibodies used include: Alexa fluor 488-tagged β1 Integrin (Santa Cruz Biotechnology, USA; Cat# sc-374429 AF488), Anti-IL-28A/IFNλ2 (Antibodies online, USA; Cat# ABIN357173), Anti-Ly6G (Antibodies online, USA; Cat# ABIN1854937), IL-29 antibody (Biorbyt, USA; Cat# orb6201), anti-IFNα (Thermo Fisher, USA; Cat# 221001), anti-Myeloperoxidase/MPO (R&D Systems, USA; Cat# AF3667-SP), anti-LCN-2 (EMD Milipore; Cat# AB2267) and anti-Actin (Sigma Aldrich, USA; Cat# A2066).

Ghosh S., Padmanabhan A., Vaidya T., Watson A.M., Bhutto I.A., Hose S., Shang P., Stepicheva N., Yazdankhah M., Weiss J., Das M., Gopikrishna S., A.i.s.h.w.a.r.y.a., Yadav N., Berger T., Mak T.W., Xia S., Qian J., Lutty G.A., Jayagopal A., Zigler JS J.r., Sethu S., Handa J.T., Watkins S.C., Ghosh A, & Sinha D. (2019). Neutrophils homing into the retina trigger pathology in early age-related macular degeneration. Communications Biology, 2, 348.

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