At the corresponding times of the respective experiments, the pH values were quantified by means of the Calimatic 766 pH meter (Knick, Berlin, Germany), both before and after the treatment of the samples with the respective CAPs. A combination pH electrode with a flat membrane (InLab-surface, Mettler-Toledo, Giessen, Germany) was used to measure the pH on solid objects (agar, bone surfaces).
Inlab surface
Manufactured by Mettler Toledo
Sourced in Germany, Switzerland
The InLab Surface is a high-quality pH/ORP electrode designed for surface measurements. It features a flat, glass-free tip for easy cleaning and maintenance. The electrode is suitable for a variety of applications, including laboratory, industrial, and environmental settings.
Automatically generated - may contain errors
Lab products found in correlation
Orion star a211, by Thermo Fisher Scientific (2 mentions)
Scanvac coolsafe, by Labogene (1 mentions)
Moisturemeterd compact, by Delfin Technologies (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
6 protocols using inlab surface
1
pH Measurement of Samples
2
Freeze-Dried Sample Analysis
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For determination of pH and dry matter, samples were freeze-dried (CoolSafe ScanVac, Labogene, Allerød, Denmark) and dry matter content calculated as the difference in weight before and after. Subsequently, the samples (1 sample of approximately 150 mg/animal), were homogenized in 145 mM KCl, 10 mM NaCl and 5 mM Na-iodoacetate (pH 7.0) in a ratio of 1:20 (sample weigh: buffer volume) and pH measured using a pH electrode (InLab Surface, Mettler Toledo, Glostrup, Denmark).
Analysis of glycogen content and lactate dehydrogenase (LDH) activity was carried out as described by Oksbjerg et al. (2019) (link).
Analysis of glycogen content and lactate dehydrogenase (LDH) activity was carried out as described by Oksbjerg et al. (2019) (link).
3
Noninvasive Skin Biometrics Monitoring
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Skin pH was tested with the potentiometrically operating electrode Inlab Surface (Mettler Toledo GmbH, Giessen, Germany) measuring at an accuracy of ± 0.01. Skin moisture in percentage was measured with a MoistureMeterD compact (Delfin Technologies UK Ltd., Dorking, UK) with a measurement accuracy of ± 5%. The measuring principle is based on noninvasive detection of the tissue dielectric constant. Both instruments were in contact with the skin and especially the measurement of skin moisture needed contact pressure. Therefore, these measurements could have an influence on microcirculation parameters. Consequently, we measured these two parameters only before and after the experiment and thus chose a contact‐free infrared thermometer FT90 (Beurer medical GmbH, Ulm, Germany) at an accuracy of ± 2°C. In accordance with literature, there were no decisive temperature‐driven changes in blood flow under 35°C expected.40 , 41 For this reason, we expected the accuracy was sufficient. The contact‐free measurement of skin temperature enabled this measurement during the experiment without an influence on microcirculation parameters. Data are provided as mean ± SD of all subjects (N = 10) per treatment duration and time of acquisition.
4
Skin pH and Microcirculation Measurement
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Skin pH was measured with the potentiometrically operating electrode Inlab Surface (Mettler Toledo GmbH, Giessen, Germany) at an accuracy of ±0.01. Since this device required direct skin contact, an impact on cutaneous microcirculation cannot be excluded. Therefore, the acquisition step (1) was followed by a stabilization phase (2). A combination unit PCE-313A (PCE Instruments, Meschede, Germany) controlled the room temperature and the relative humidity throughout all experiments. With room temperature of 23.8 ± 0.9°C and relative air humidity of 38.3 ± 5.1%, climatic conditions were thought to be sufficiently stable to ensure comparability of the microcirculation results.
5
pH Measurement of Solid Specimens
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The pH was measured by using a pH meter (ORION STAR A211; Thermo Scientific, MA, USA) with an electrode for solid specimens (InLab Surface; Mettler Toledo, Schwerzenbach, Switzerland). Before the test, the apparatus was calibrated at the standard pH solutions of 4.0, 7.0 and 11.0. Six specimens for each group were prepared by loading the material under test into acrylic molds with 10.0 mm inner diameter and 5.0 mm height (n=6). The readings were taken at the end of the mixing and after 30 m, 1 h, 3 h, 24 h, 48 h and 72 h. Between each measurement, the electrode was washed with distilled water and blot dried.
6
pH Evaluation of Solid Biomaterials
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The pH was measured by using a pH meter (ORION STAR A211, Thermo Scientific, Waltham, MA, USA) with an electrode for solid specimens (InLab Surface, Mettler Toledo, Schwerzenbach, Switzerland). Before the test, the apparatus was calibrated with standard solutions of pH 4.0, 7.0, and 11.0. Six samples (10.0 mm in diameter and 5.0 mm in height) of each material were used. Readings were taken at various time intervals after setting (after 3 h, 24 h, 7 days, and 14 days). Between each measurement, the electrode was washed with distilled water and blot dried.
Cell culture MC3T3-E1 cells were used for this study. They were cultured in Dulbecco's modified Eagle's medium (DMEM; Invitrogen, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (FBS; Invitrogen) and 1% antibiotics (100 U/mL of penicillin and 100 μg/mL of streptomycin, Invitrogen) at 37ºC in a humidified atmosphere containing 5% CO 2.
Cell culture MC3T3-E1 cells were used for this study. They were cultured in Dulbecco's modified Eagle's medium (DMEM; Invitrogen, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (FBS; Invitrogen) and 1% antibiotics (100 U/mL of penicillin and 100 μg/mL of streptomycin, Invitrogen) at 37ºC in a humidified atmosphere containing 5% CO 2.
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