Log In Sign up
The largest database of trusted experimental protocols

Cd45 pe dazzle594

Manufactured by BioLegend
Visit Supplier

CD45-PE-Dazzle594 is a fluorochrome-conjugated antibody that binds to the CD45 antigen. CD45 is a transmembrane glycoprotein expressed on the surface of all leukocytes. The PE-Dazzle594 fluorochrome provides a distinct signal that can be detected using flow cytometry.

Automatically generated - may contain errors

Lab products found in correlation

Zombie uv dye, by BioLegend (7 mentions) Tnfα bv650, by BioLegend (3 mentions) Cd11b ef450, by BioLegend (3 mentions) Human trustain fcx, by BioLegend (2 mentions) Nk1.1 bv711, by BioLegend (2 mentions) Cd8 bv510, by BioLegend (2 mentions) Mp6 xt22, by BioLegend (2 mentions) B220 bv605, by BioLegend (2 mentions) Il 17a percp cy5, by Thermo Fisher Scientific (1 mentions) Ifn γ pe cy7, by Thermo Fisher Scientific (1 mentions) Rm4 5, by Thermo Fisher Scientific (1 mentions) H57 597, by Thermo Fisher Scientific (1 mentions) F4 80 apc, by Thermo Fisher Scientific (1 mentions) Cd11c bv711, by BioLegend (1 mentions) Ly6c percp cy5, by Thermo Fisher Scientific (1 mentions) Zombie uv live dead dye, by BioLegend (1 mentions) F4 80 af700, by BioLegend (1 mentions) Nk1.1 fitc, by BioLegend (1 mentions) Lsrfortessa, by BD (1 mentions) Dnase 1, by Roche (1 mentions)

Close spelling variants of this product

CD45-PE

11 protocols using cd45 pe dazzle594

1

Single-Cell Isolation of Immune Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Tumor and adjacent normal tissue cells and peripheral blood mononuclear cells (PBMCs) were resuspended in FACS staining buffer (1% bovine serum albumin (BSA) and 1 mM EDTA in Dulbecco’s phosphate-buffered saline (PBS); Gibco) and incubated with Human TruStain FcX (BioLegend) for 10 min on ice to block nonspecific binding to Fc receptors. Cells were then washed and stained with CD45-PE-Dazzle594 (BioLegend) for 20 min on ice. Next, cells were filtered and resuspended in FACS staining buffer with addition of DNase for FACS sorting. DAPI was added to the cell suspension immediately before FACS sorting for dead cell exclusion. Live CD45+ single cells were sorted for downstream single-cell analysis.
Kourtis N., Wang Q., Wang B., Oswald E., Adler C., Cherravuru S., Malahias E., Zhang L., Golubov J., Wei Q., Lemus S., Ni M., Ding Y., Wei Y., Atwal G.S., Thurston G., Macdonald L.E., Murphy A.J., Dhanik A., Sleeman M.A., Tykodi S.S, & Skokos D. (2022). A single-cell map of dynamic chromatin landscapes of immune cells in renal cell carcinoma. Nature Cancer, 3(7), 885-898.
+ Open protocol
+ Expand
2

Cytokine Profiling of Immune Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mouse cells were stained with Live/Dead stain (Zombie UV Dye: Biolegend) and with directly conjugated monoclonal antibodies to CD45-PE-Dazzle594 (Biolegend, 104), NK1.1-BV711 (Biolegend, PK136) then fixed, permeabilized and stained for the cytokine IFNγ-PE-Cy7 (e-Biosciences, XMG1.2).
Oates J.R., Sawada K., Giles D.A., Alarcon P.C., Damen M.S., Szabo S., Stankiewicz T.E., Moreno-Fernandez M.E, & Divanovic S. (2023). Thermoneutral housing shapes hepatic inflammation and damage in mouse models of non-alcoholic fatty liver disease. Frontiers in Immunology, 14, 1095132.
+ Open protocol
+ Expand
3

Multiparameter Flow Cytometry of Immune Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mouse cells were stained with Live/Dead stain (Zombie UV Dye: Biolegend) and with directly-conjugated monoclonal antibodies to CD45-PE-Dazzle594 (Biolegend, 104), TCRβ-APCef780 or APC (Invitrogen, H57-597), CD8-BV510 (Biolegend, 53-6.7), then fixed, permeabilized and stained for the cytokines IL-17A-PerCpCy5.5 or PE (e-Biosciences, 17B7), IFNγ-PE-Cy7 (e-Biosciences, XMG1.2) and TNFα-BV650 (Biolegend, MP6-XT22).
Oates J.R., Sawada K., Giles D.A., Alarcon P.C., Damen M.S., Szabo S., Stankiewicz T.E., Moreno-Fernandez M.E, & Divanovic S. (2023). Thermoneutral housing shapes hepatic inflammation and damage in mouse models of non-alcoholic fatty liver disease. Frontiers in Immunology, 14, 1095132.
+ Open protocol
+ Expand
4

Multiparameter Cell Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mouse cells were stained with Live/Dead stain (Zombie UV Dye: Biolegend) and with directly conjugated monoclonal antibodies to CD45-PE-Dazzle594 (Biolegend, 104) and B220-BV605 (Biolegend, RA3-6B2).
Oates J.R., Sawada K., Giles D.A., Alarcon P.C., Damen M.S., Szabo S., Stankiewicz T.E., Moreno-Fernandez M.E, & Divanovic S. (2023). Thermoneutral housing shapes hepatic inflammation and damage in mouse models of non-alcoholic fatty liver disease. Frontiers in Immunology, 14, 1095132.
+ Open protocol
+ Expand
5

Multiparameter Flow Cytometric Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mouse cells were stained with Live/Dead stain (Zombie UV Dye: Biolegend) and with directly-conjugated monoclonal antibodies to CD45-PE-Dazzle594 (Biolegend, 104), TCRβ-APCef780 or APC (Invitrogen, H57-597), CD4-APC-ef780 or BV786 (e-Biosciences, RM4-5), then fixed, permeabilized and stained for the cytokines IL-17A-PerCpCy5.5 or PE (e-Biosciences, 17B7), IFNγ-PE-Cy7 (e-Biosciences, XMG1.2) and TNFα-BV650 (Biolegend, MP6-XT22).
Oates J.R., Sawada K., Giles D.A., Alarcon P.C., Damen M.S., Szabo S., Stankiewicz T.E., Moreno-Fernandez M.E, & Divanovic S. (2023). Thermoneutral housing shapes hepatic inflammation and damage in mouse models of non-alcoholic fatty liver disease. Frontiers in Immunology, 14, 1095132.
+ Open protocol
+ Expand
6

Multicolor flow cytometry of mouse cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mouse cells were stained with Live/Dead stain (Zombie UV Dye: Biolegend) and with directly conjugated monoclonal antibodies to CD45-PE-Dazzle594 (Biolegend, 104), NK1.1-BV711 (Biolegend, PK136) and TCRβ-APCef780 or APC (Invitrogen, H57-597).
Oates J.R., Sawada K., Giles D.A., Alarcon P.C., Damen M.S., Szabo S., Stankiewicz T.E., Moreno-Fernandez M.E, & Divanovic S. (2023). Thermoneutral housing shapes hepatic inflammation and damage in mouse models of non-alcoholic fatty liver disease. Frontiers in Immunology, 14, 1095132.
+ Open protocol
+ Expand
7

Multicolor Flow Cytometric Profiling of Immune Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mouse cells were stained with Live/Dead stain (Zombie UV Dye: Biolegend) and with directly conjugated monoclonal antibodies to CD45-PE-Dazzle594 (Biolegend, 104), CD11b-eF450 (Biolegend, 17A2), F4/80-APC (eBiosciences, BM8) then fixed, permeabilized and stained for the cytokine TNFα-BV650 (Biolegend, MP6-XT22).
Oates J.R., Sawada K., Giles D.A., Alarcon P.C., Damen M.S., Szabo S., Stankiewicz T.E., Moreno-Fernandez M.E, & Divanovic S. (2023). Thermoneutral housing shapes hepatic inflammation and damage in mouse models of non-alcoholic fatty liver disease. Frontiers in Immunology, 14, 1095132.
+ Open protocol
+ Expand
8

Multiparametric Immune Cell Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mouse cells were stained with Live/Dead stain (Zombie UV Dye: Biolegend) and with directly conjugated monoclonal antibodies to CD45-PE-Dazzle594 (Biolegend, 104), CD11b-eF450 (Biolegend, 17A2), Gr1-FITC (Invitrogen, RB6-8C5) then fixed, permeabilized and stained for the cytokine TNFα-BV650 (Biolegend, MP6-XT22).
Oates J.R., Sawada K., Giles D.A., Alarcon P.C., Damen M.S., Szabo S., Stankiewicz T.E., Moreno-Fernandez M.E, & Divanovic S. (2023). Thermoneutral housing shapes hepatic inflammation and damage in mouse models of non-alcoholic fatty liver disease. Frontiers in Immunology, 14, 1095132.
+ Open protocol
+ Expand
9

Isolation and Characterization of Uterine Immune Cells

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Uterine samples consisting of maternal decidua and myometrium were
collected 6 h after ultrapure LPS challenge (or saline) on d16 of gestation
from WT and BAFF reporter (RFP48 (link)) mice. Immune cells were isolated using enzymatic
digestion. Briefly, uterine tissue was dissected and finely minced. Tissue
was digested using Liberase TM (21 µg/mL, Roche) and Dnase I (8.8
µg/mL, Roche) in DMEM containing HEPES and 2% BSA. Tissue was
incubated at 37°C for 30 min at 220 RPM. After digestion, cells were
filtered through a 100 µM strainer and centrifuged at 800 g for 5
min. Following red blood cell lysis, single cell suspensions were stained
with Live/Dead (Zombie UV Dye: Biolegend) and directly conjugated monoclonal
antibodies to CD45-PE-Dazzle594 (Biolegend, 104), F4/80-AF700 (Biolegend,
BM8), TCRb-APC-ef780 (Invitrogen, H57–597), CD11b-ef450 (Biolegend,
17A2), CD8-BV510 (Biolegend, 53–6.7), B220-BV605 (Biolegend,
RA3–6B2), NK1.1-FITC (Biolegend, PK136), CD11c-BV711 (Biolegend,
N418), Ly6G-APC (Invitrogen, RB6–8C5), Ly6C-PerCP-Cy5.5 (eBioscience,
HK1.4). Data was collected using LSR Fortessa (BD) and analyzed using FlowJo
X software (vX10).
Smith H.C., Kuo S.R., Backus J.W., Harris S.G., Sparks C.E, & Sparks J.D. (1991). In vitro apolipoprotein B mRNA editing: identification of a 27S editing complex.. Proceedings of the National Academy of Sciences of the United States of America, 88(4), 1489-1493.
+ Open protocol
+ Expand
10

Isolation of Live CD45+ Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Single-cell dissociated from human primary tumors and PBMCs were resuspended in the PBS and incubated with Human TruStain FcX (BioLegend, cat. no. 422301, RRID:AB_2818986) for 10 minutes on ice to block nonspecific Fc receptors binding. The cells were then washed and stained with CD45-PE-Dazzle594 (BioLegend, cat. no. 260178, RRID:AB_2928006) and Fixable Viability Dye eFluor 780 (Thermo, cat. no. 65-0865-18) for 45 minutes on ice. Next, cells were washed, filtered, and resuspended in the PBS buffer for FACS sorting. Live CD45+ single cells were sorted.
Ran X., Zheng J., Chen L., Xia Z., Wang Y., Sun C., Guo C., Lin P., Liu F., Wang C., Zhou J., Sun C., Liu Q., Ma J., Qin Z., Zhu X, & Xie Q. (2023). Single-Cell Transcriptomics Reveals the Heterogeneity of the Immune Landscape of IDH–Wild-Type High-Grade Gliomas. Cancer Immunology Research, 12(2), 232-246.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!