Rabbit antibody to rfp

Manufactured by Thermo Fisher Scientific

The Rabbit antibody to RFP is a primary antibody that specifically binds to the red fluorescent protein (RFP) commonly used as a reporter or fusion tag in biological research. This antibody can be used to detect and visualize RFP-tagged proteins in various applications, such as Western blotting, immunocytochemistry, and immunohistochemistry.

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Lab products found in correlation

Alexa633 conjugated, by Thermo Fisher Scientific (2 mentions) Mouse antibody to myc 4e10, by Santa Cruz Biotechnology (2 mentions) Rabbit antibody to gfp conjugated with alexa488, by Thermo Fisher Scientific (2 mentions) Alexa 488, by Thermo Fisher Scientific (2 mentions) Alexa 568, by Thermo Fisher Scientific (1 mentions)

2 protocols using rabbit antibody to rfp

Drosophila Brain Immunostaining and Imaging

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The experimental procedures for brain dissection, fixation, and immunostaining as well as agarose section were as described previously (Hakeda-Suzuki et al., 2011 (link)). The following primary antibodies were used: mAb24B10 (1:50, DSHB), rat antibody to CadN (Ex#8, 1:50, DSHB), mouse antibody to Repo (8D12, 1:20, DSHB) mouse antibody to myc (4E10, 1:100, Santa Cruz), rabbit antibody to RFP (1:500 ROCKLAND), rabbit antibody to GFP conjugated with Alexa488 (1:200, Life technologies). The secondary antibodies were Alexa488, Alexa568, or Alexa633-conjugated (1:400, Life technologies). Images were obtained with Nikon C2⁺ and A1 confocal microscopes and processed with Adobe Photoshop and Illustrator.
Live imaging was done according to Özel et al., 2015 (link). Images were obtained with Zeiss LSM880NLO + COHERENT Chameleon Vision.

Takechi H., Hakeda-Suzuki S., Nitta Y., Ishiwata Y., Iwanaga R., Sato M., Sugie A, & Suzuki T. (2021). Glial insulin regulates cooperative or antagonistic Golden goal/Flamingo interactions during photoreceptor axon guidance. eLife, 10, e66718.

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Brain Dissection and Immunostaining Protocol

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The experimental procedures for brain dissection, fixation and immunostaining as well as agarose section were as described previously (Hakeda-Suzuki et al., 2011) . The following primary antibodies were used: mAb24B10 (1:50, DSHB), rat antibody to CadN (Ex#8, 1:50, DSHB), mouse antibody to Repo (8D12, 1:20, DSHB) mouse antibody to myc (4E10, 1:100, Santa Cruz), rabbit antibody to RFP (1:500 ROCKLAND), rabbit antibody to GFP conjugated with Alexa488 (1:200, Life technologies). The secondary antibodies were Alexa488, Alexa568 or Alexa633-conjugated (1:400, Life technologies). Images were obtained with Nikon C2 + and A1 confocal microscopes and processed with Adobe Photoshop and Illustrator.
(which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission.
The copyright holder for this preprint this version posted January 24, 2020. ; https://doi.org/10.1101/2020.01.23.916403 doi: bioRxiv preprint 7
Live imaging was done according to (Özel et al., 2015) . Images were obtained with Zeiss LSM880NLO + COHERENT Chameleon Vision.

Takechi H., Hakeda‐Suzuki S., Nitta Y., Ishiwata Y., Sato M., Sugie A., & Suzuki T. (2020). Glial insulin regulates cooperative or antagonistic Golden goal/Flamingo interactions during photoreceptor axon navigation.

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