Trp53flox

Manufactured by Jackson ImmunoResearch

The Trp53flox/+ mouse model is a genetically engineered mouse strain that carries a floxed (flanked by loxP sites) allele of the Trp53 gene. The Trp53 gene encodes the p53 protein, which plays a crucial role in regulating cell growth and division. This mouse model allows for the conditional deletion or inactivation of the Trp53 gene in a tissue-specific or cell-type-specific manner, enabling the study of p53 function in various biological processes.

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Lab products found in correlation

Lsl krasg12d, by Jackson ImmunoResearch (1 mentions) Pdx1 cre mice, by Jackson ImmunoResearch (1 mentions) Etv1cre er t2, by Jackson ImmunoResearch (1 mentions) Brafca, by Jackson ImmunoResearch (1 mentions)

2 protocols using trp53flox

Kras, Trp53, and Pdx1 Driven Pancreatic Tumor Development

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Three individual strains of LSL-Kras^G12D/+, Trp53^flox/+, and Pdx-1-cre mice were obtained from Jackson Laboratory (Sacramento). We crossed and generated the LSL-Kras^G12D/+;Trp53^flox/+;Pdx-1-cre (KPC^flox) mice in house. All animals were kept in specific pathogen-free housing with abundant food and water under guidelines approved by the Nihon University, School of Medical, Animal Care and Use Committee (AP15M001). At 3 months old, the mice were intraperitoneally injected with 40 mg/kg Indox or a vehicle (a mixture of DMSO/PEG400, 1:1 (v/v)) twice a week until the endpoint. The total pancreatic weight and tumor sizes were measured, and their volumes were calculated using the following formula: width x length x height. All samples were embedded in paraffin followed after fixing in 10% neutral buffered formalin.

Ichimaru Y., Sano M., Kajiwara I., Tobe T., Yoshioka H., Hayashi K., Ijichi H, & Miyairi S. (2019). Indirubin 3′-Oxime Inhibits Migration, Invasion, and Metastasis InVivo in Mice Bearing Spontaneously Occurring Pancreatic Cancer via Blocking the RAF/ERK, AKT, and SAPK/JNK Pathways. Translational Oncology, 12(12), 1574-1582.

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Genetically Engineered Mouse Models for GI Tumors

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All mouse studies are conducted in accordance with MSKCC IACUC under protocol 11-12-029. The Braf^CA, Etv1^CreERT2, Trp53^flox and Rosa26^{CAGGS-LSL-EYFP} mice were obtained from The Jackson Laboratory. To trace the Etv1-expressing cellular compartments in the GI tract, Etv1^CreERT2/+; Rosa26^{CAGGS-LSL-EYFP/+} mice were generated through standard mouse breeding with Etv1^{CreERT2/CreERT2} and Rosa26^{CAGGS-LSL-EYFP/CAGGS-LSL-EYFP} mice. Etv1^CreERT2; Braf^CA/CA and Etv1^CreERT2; Braf^CA/+ mice were generated through breeding of Etv1^CreERT2 and Braf^CA/CA mice. To cross into the Trp53 conditional background, we crossed Etv1^CreERT2;Braf^CA/CA with Trp53^flox mice to generate Etv1^CreERT2;Braf^CA/+;Trp53^flox/flox and Etv1^CreERT2;Braf^CA/CA;Trp53^flox/flox compound genetically engineered mice.

Ran L., Murphy D., Sher J., Cao Z., Wang S., Walczak E., Guan Y., Xie Y., Shukla S., Zhan Y., Antonescu C.R., Chen Y, & Chi P. (2017). ETV1-positive cells give rise to BRAFV600E mutant gastrointestinal stromal tumors. Cancer research, 77(14), 3758-3765.

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