Fitc conjugated cd44

MB109 is a recombinantly expressed human BMP-9 cytokine. It contains a methionine residue in front of the mature domain of human BMP-9 (Ser320-Arg429, 110 residues). MB109 was prepared as described previously [13 ]. Recombinant BMP-2 was purchased from joint Protein Central (jointproteincentral.com, Incheon, Korea). The antibodies against p21, P-SMAD1/5/8 and P-p38 were purchased from Cell Signaling Technology (Massachusetts, USA), ID3 from Santa Cruz Biotechnology (California, USA), β-actin from Sigma Aldrich (Missouri, USA), CD90 from Novus Biologicals (CO, USA), AFP from Abcam (Cambridge, UK), PE-conjugated CD90 from BD Biosciences (New Jersey, USA) and FITC-conjugated CD44 from Miltenyi Biotec (Bergisch Gladbach, Germany). Chemical inhibitors, LDN193189 was purchased from Sellekchem (Texas, USA) and SB202190 was purchased from Sigma Aldrich (Missouri, USA). The cell culture media, fetal bovine serum (FBS), Penicillin-Streptomycin Solution (P/S) and Trypsin-EDTA were purchased from Hyclone (Utah, USA).

Rabbit monoclonal anti-NANOG (CAT: 4903S), anti-SOX2 (CAT: 3579S), anti-OCT4 (CAT: 2750S) and anti-GAPDH (CAT: 5174S) used for western blot assays were purchased from Cell Signaling Technology (CST, US). Goat anti-rabbit IgG-HRP was obtained from Santa Cruz Biotechnology (Santa Cruz, US). Antibodies to FITC-conjugated CD44 (CAT: 130-095-195) and PE-conjugated CD24 (CAT: 130-095-953) used for Fluorescence-activated cell sorting (FACS) were ordered from Miltenyi Biotec (US).