Cellular protein was lysed by radio immune precipitation assay (RIPA) buffer (Beyotime, China) with phenylmethane sulfonyl fluoride (PMSF) protease inhibitor (Beyotime, China) and the homogenate was centrifuged at 12,000 × g for 5 min at 4 ◦C. The supernatant was collected and the protein concentration was determined immediately using a BCA protein quantification kit (Beyotime, China). The proteins were separated in 10% SDS-PAGE and transferred onto PVDF membrane, and then probed with antibodies following standard procedures. The antibodies and their dilutions utilized for western blots were as follow: anti-GHR (bs-0654R; Bioss, China; 1:500), anti-PGC1α (bs-1832R; Bioss, China; 1:500), anti-NRF1 (12,482–1-AP; Proteintech, USA; 1:500), anti-TOMM20 (AF1717; Beyotime, China; 1:500), anti-JAK2 (bs-0908R; Bioss, China; 1:1000), anti-p-JAK2 (bsm-52171R; Bioss, China; 1:1000), anti-AKT1 (bs-0115 M; Bioss, China; 1:500), anti-p-AKT1 (66,444–1-Ig; Proteintech, China; 1:500), anti-CREB1 (bs-0035R; Bioss, China; 1:500), anti-p-CREB1 (bs-0036R; Bioss, China; 1:500), anti-β-actin (bs-0061R; Bioss, China; 1:1000), goat anti-rabbit IgG-HRP (bs-0295G; Bioss, China; 1:5000), goat anti-mouse IgG-HRP (bs-0296G; Bioss, China; 1:5000).
Anti pgc1α bs 1832r
Manufactured by Bioss Antibodies
Sourced in China
Anti-PGC1α (bs-1832R) is a research-use antibody product offered by Bioss Antibodies. It is designed to detect the Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha (PGC1α) protein, which is involved in the regulation of mitochondrial biogenesis and energy metabolism.
Automatically generated - may contain errors
Lab products found in correlation
Bca protein quantification kit, by Beyotime (1 mentions)
Protease inhibitor pmsf, by Beyotime (1 mentions)
Anti p akt1, by Proteintech (1 mentions)
Anti β actin bs 0061r, by Bioss Antibodies (1 mentions)
Goat anti mouse igg hrp, by Bioss Antibodies (1 mentions)
Bs 0296g, by Bioss Antibodies (1 mentions)
Ripa buffer, by Beyotime (1 mentions)
2 protocols using anti pgc1α bs 1832r
1
Western Blot Analysis of Cellular Signaling
2
Muscle Growth Regulatory Factors
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Protein abundances of some key regulatory factors involved in the growth and development of muscle were determined by the standard western blot method with the GAPDH protein as a loading control. The anti-MyoD (#13812), anti-INSR(#74118) and anti-GAPDH (#2118) primary antibodies and horseradish peroxidase-conjugated secondary antibodies (anti-rabbit IgG, #5127; anti-Mouse IgG, # 58820) were obtained from CST (Cell Signaling, MA, USA). The anti-MYF5 (# bs-6936R) and anti-PGC 1α (# bs-1832R) primary antibodies were obtained from Bioss (Bioss Biotech, Beijing, China). The anti-myogenin (MyoG, # ab1835) primary antibody was obtained from Abcam Biotech (Abcam Biotech, Cambridge, UK). The anti-IGF1R (#20254-1-AP), anti-AKT (#10176-2-AP), anti-P-AKT (#66444-1-Ig), anti-mTOR (#66888-1-Ig), anti-P-mTOR (#67778-1-Ig) primary antibodies were obtained from Proteintech (Proteintech Biotech, Wuhan, China).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!