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Fcf 200 cu

Manufactured by Electron Microscopy Sciences
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Sourced in United States, United Kingdom

The FCF-200-Cu is a compact and versatile fluorescent camera designed for electron microscopy applications. It features a high-resolution CMOS sensor and a robust copper housing, providing reliable performance and durability for demanding imaging tasks.

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Lab products found in correlation

Jem 1400, by JEOL (5 mentions) 1010 microscope, by JEOL (3 mentions) Ht7700 transmission electron microscope, by Hitachi (2 mentions) Jem 2000exii, by JEOL (2 mentions) Zetasizer nano z, by Malvern Panalytical (2 mentions) Tio2 np, by Merck Group (2 mentions) Ab59479, by BD (2 mentions) Ab2413, by Abcam (2 mentions) Ab34710, by Abcam (2 mentions) Sab 4200476, by Merck Group (2 mentions) Mab 2679, by Cell Signaling Technology (2 mentions) Nano w stain, by Nanoprobes (1 mentions) Amt xr11 digital camera, by Philips (1 mentions) Whatman 1 filter paper, by Cytiva (1 mentions) Cm12 electron microscope, by Philips (1 mentions) Formvar coated 200 mesh copper grid, by Electron Microscopy Sciences (1 mentions) 100 cxii electron microscope, by JEOL (1 mentions) Filter paper, by Cytiva (1 mentions) Tecnai t12 tem, by Thermo Fisher Scientific (1 mentions) 2010f tem stem, by JEOL (1 mentions)

32 protocols using fcf 200 cu

1

Characterizing AuNP Core Size and Shape

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The core size and shape of AuNP were examined with transmission electron microscopy (TEM) using a JEOL 1010 microscope (JEOL USA Inc., Peabody, MA) at 80 kV. 10 μL of diluted AuNP solution was dropped onto carbon-coated copper grids (FCF-200-Cu, Electron Microscopy Sciences, Hatfield, PA). The solution was allowed to evaporate before performing microscopy. The average core size of AuNP was determined by manually measuring particles using ImageJ (National Institutes of Health).
Dong Y.C., Hajfathalian M., Maidment P.S., Hsu J.C., Naha P.C., Si-Mohamed S., Breuilly M., Kim J., Chhour P., Douek P., Litt H.I, & Cormode D.P. (2019). Effect of Gold Nanoparticle Size on Their Properties as Contrast Agents for Computed Tomography. Scientific Reports, 9, 14912.
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2

TEM Imaging of Dex-NZM Nanoparticles

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Transmission electron microscopy (TEM) of each Dex-NZM was performed using a JEOL 1010 microscope operating at 80 kV. Then 5 μL of nanoparticle suspension was dropped onto the TEM grid (FCF-200-Cu, Electron Microscopy Sciences, Hatfield, PA), and the liquid was allowed to dry before microscopy was performed.
Naha P.C., Liu Y., Hwang G., Huang Y., Gubara S., Jonnakuti V., Simon-Soro A., Kim D., Gao L., Koo H, & Cormode D.P. (2019). Dextran-Coated Iron Oxide Nanoparticles as Biomimetic Catalysts for Localized and pH-Activated Biofilm Disruption. ACS nano, 13(5), 4960-4971.
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3

Visualizing Extracellular Vesicle Morphology

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Fractions most enriched with PKD-2::GFP carrying EVs were diluted 10–20 times with M9 buffer and applied to discharged formvar/carbon coated copper grids (200 mesh, Electron Microscopy Sciences # FCF200-Cu). Five microliters of the diluted fraction were dispensed on the grid and allowed on for 30–60 seconds followed by wicking the liquid away from the grid with Whatman filter paper #1. The grid was then transferred through 3 droplets of PBS (5 min in each droplet) to wash away residual sucrose and iodixanol. The excess of PBS was wicked away with the filter paper after each wash. For the unfixed preparation, following PBS washes the grid was exposed to the Nano-W stain (Methylamine Tungstate solution, Nanoprobes #2018) for 1 minute sharp. The stain was removed with filter paper and the grid was left to dry out completely for 30 minutes at room temperature. Imaging was performed on Philips CM12 electron microscope with AMT-XR11 digital camera. Best results were achieved with imaging the samples on the day of their staining.
Nikonorova I.A., Wang J., Cope A.L., Tilton P.E., Power K.M., Walsh J.D., Akella J.S., Krauchunas A.R., Shah P, & Barr M.M. (2022). Isolation, profiling, and tracking of extracellular vesicle cargo in Caenorhabditis elegans. Current biology : CB, 32(9), 1924-1936.e6.
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4

Electron Microscopy Sample Preparation

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Samples were thawed and diluted in labeling buffer (20 mM sodium phosphate [pH 7.4], 50 mM KCl, 5 mM MgCl2) to 20 μM, and 5 μL was applied to a Formvar-coated 200-mesh copper grid (catalog number FCF200-CU; Electron Microscopy Sciences). Samples were air dried for 15 min, and the excess solution was wicked off. Salt was removed by three 5-μL additions of deionized water before staining with 3 μL of 2% uranyl acetate for 10 s. Samples were imaged using a JEOL 100CXII electron microscope.
Trettel D.S, & Winkler W.C. (2023). In Vitro Analysis of Bacterial Microcompartments and Shell Protein Superstructures by Confocal Microscopy. Microbiology Spectrum, 11(2), e03357-22.
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5

TEM Analysis of Metal Particles

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Particle size and shape were assessed by TEM. 5-μl droplets of the metal particle dispersions were pipetted on formvar/carbon 200 mesh grids (FCF200-CU, Electron Microscopy Sciences, Hatfield, PA), and dried by removing excess water using a filter paper (Whatman, Little Chalfont, UK). TEM imaging was performed using a transmission electron microscope (JEM-1400, JEOL, Tokyo, Japan) operated at 80 keV acceleration voltage in bright-field mode.
Jämsen E., Pajarinen J., Kouri V.P., Rahikkala A., Goodman S.B., Manninen M., Nordström D.C., Eklund K.K, & Nurmi K. (2020). Tumor necrosis factor primes and metal particles activate the NLRP3 inflammasome in human primary macrophages. Acta biomaterialia, 108, 347-357.
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6

Extracellular Vesicle Imaging by TEM

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EVs were dropped onto formvar/carbon 200 mesh copper grids (FCF200-CU; Electron Microscopy Sciences) and incubated for 5 min at room temperature. The grid was washed with the filtrated deionized water, negatively stained using 2% uranyl acetate (#22400; Electron Microscopy Sciences) in 50% methanol for 1 min and then air-dried at room temperature. Imaging was visualized using HT 7700 transmission electron microscope (Hitachi, Krefeld, Germany) with an acceleration voltage of 100 kV at the Faculty of Tropical Medicine, Mahidol University, Thailand.
Panachan J., Rojsirikulchai N., Pongsakul N., Khowawisetsut L., Pongphitcha P., Siriboonpiputtana T., Chareonsirisuthigul T., Phornsarayuth P., Klinkulab N., Jinawath N., Chiangjong W., Anurathapan U., Pattanapanyasat K., Hongeng S, & Chutipongtanate S. (2022). Extracellular Vesicle-Based Method for Detecting MYCN Amplification Status of Pediatric Neuroblastoma. Cancers, 14(11), 2627.
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7

Negative Staining of Purified LEVs and SEVs

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For negative staining of purified LEVs and SEVs, Formvar carbon film–coated grids (FCF-200-Cu; Electron Microscopy Sciences) were washed in double distilled water and then washed by 100% ethanol. For each step, excess liquid was removed by wicking with filter paper. 10-μl samples were added to grids for 2 min. Grids were immediately stained with 2% phosphotungstic acid, pH 6.1, for 30s and allowed to air-dry. Grids were imaged using a FEI Tecnai T12 TEM (120 kV LaB6 source), Gatan cryotransfer stage, and AMT XR41-S side-mounted 2K × 2K CCD camera, 2102 SC.
Jimenez L., Yu H., McKenzie A.J., Franklin J.L., Patton J.G., Liu Q, & Weaver A.M. (2019). Quantitative proteomic analysis of small and large extracellular vesicles (EVs) reveals enrichment of adhesion proteins in small EVs.. Journal of proteome research, 18(3), 947-959.
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8

Liposome Morphology Characterization via TEM

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Liposome morphology was observed by transmission electron microscopy (TEM) to characterize the microstruce. The liposome suspension was dropped onto a Formvar/carbon film on a 200-mesh copper grid (FCF-200-Cu; Electron Microscopy Sciences, Hatfield, PA, USA) and stained with 1% phosphotungstic acid for 20 minutes. After the negative-staining process, the sample was washed with distilled deionized water. The water was vacuumed out overnight, and the sample was then observed using TEM (JEM-2000EX II; JEOL, Tokyo, Japan).
Fang Y.P., Chuang C.H., Wu Y.J., Lin H.C, & Lu Y.C. (2018). SN38-loaded <100 nm targeted liposomes for improving poor solubility and minimizing burst release and toxicity: in vitro and in vivo study. International Journal of Nanomedicine, 13, 2789-2802.
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9

Transmission Electron Microscopy of BION Formulations

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Transmission electron microscopy of each BION formulation was performed using a JEOL 1010 microscope operating at 80 kV. Briefly, 10 μl of diluted BION (10 μl from stock was diluted with 1 ml of DI water) was dropped onto carbon coated copper grid (FCF-200-Cu, Electron Microscopy Sciences, Hatfield, PA, USA), and allowed to evaporate before imaging. High-resolution images and SAED patterns were collected at 200 kV on a JEOL 2010F TEM/STEM and a JEOL 2100 TEM, respectively. Samples were mounted from suspension onto 200 mesh Cu grids with lacy carbon films.
Naha P.C., Zaki A.A., Hecht E., Chorny M., Chhour P., Blankemeyer E., Yates D.M., Witschey W.R., Litt H.I., Tsourkas A, & Cormode D.P. (2014). Dextran coated bismuth-iron oxide nanohybrid contrast agents for computed tomography and magnetic resonance imaging. Journal of materials chemistry. B, Materials for biology and medicine, 2(46), 8239-8248.
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10

Visualizing Extracellular Vesicles by TEM

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Transmission electronic microscopy was utilized to visualize purified EV samples by negative staining as previously described22 (link). Briefly, formvar carbon film-coated grids (FCF-200-Cu; Electron Microscopy Sciences) were placed on top of 7–10 μL of sample for 1–5 minutes. The grids were then stained with 2% phosphotungistic acid, pH 6.1 for 10–20 seconds and imaged with a FEI Tecnai T12 transmission electron microscope (120 kV LaB6 source), Gatan cryotransfer stage, and AMT XR41-S side mounted 2Kx2K CCD camera, 2102 SC.
Lim A.R., Vincent B.G., Weaver A.M, & Rathmell W.K. (2021). Sunitinib and Axitinib Increase Secretion and Glycolytic Activity of Small Extracellular Vesicles in Renal Cell Carcinoma. Cancer gene therapy, 29(6), 683-696.
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