Mice were housed in metabolic cages for 24 hours in order to obtain 24-hour urine for the measurement of Urinary Albumin Excretion (UAE). The urine volume collected per mouse was documented as urinary output in grams. UAE was measured using the Mouse albumin ELISA quantification kit (Bethyl Laboratories, TX, USA, cat no E90-134, ITK Diagnostics). UAE was corrected for urinary output per mouse. Urinary glucose was determined by a spectrophotometric measurement of glucohexokinase activity. This automated measurement was performed in the Roche cobas c702 Chemistry Analyzer at the Laboratory for General Clinical Chemistry (LAKC) at the Academic Medical Center of Amsterdam.
Mouse albumin elisa quantification kit
Manufactured by Fortis Life Sciences
Sourced in United States
The Mouse Albumin ELISA Quantification Kit is a laboratory instrument used to measure the concentration of albumin, a protein found in mouse blood serum or plasma. It employs the enzyme-linked immunosorbent assay (ELISA) technique to quantify the amount of mouse albumin present in a given sample.
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Lab products found in correlation
Quantichrom creatinine assay kit, by BioAssay Systems (3 mentions)
Quantichrom urea assay kit, by BioAssay Systems (2 mentions)
Mouse cystatin c elisa kit, by BioVendor (2 mentions)
Cobas c702 chemistry analyzer, by Roche (1 mentions)
Euthatal, by Merck Group (1 mentions)
Creatinine assay kit, by Abcam (1 mentions)
Mouse multiplex kits, by Thermo Fisher Scientific (1 mentions)
Bio plex multiplex 200 luminex reader, by Bio-Rad (1 mentions)
Creatinine assay kit, by Cayman Chemical (1 mentions)
Creatinine anhydrous, by Merck Group (1 mentions)
Douset elisa, by R&D Systems (1 mentions)
Enzymatic assay kit, by Fujifilm (1 mentions)
Dict 500, by BioAssay Systems (1 mentions)
Male apoe mice, by Charles River Laboratories (1 mentions)
Enspire multimode plate reader, by PerkinElmer (1 mentions)
Metabolic cage, by Tecniplast (1 mentions)
Detect x serum creatinine detection kit, by Arbor Assays (1 mentions)
Creatinine parameter assay kit, by R&D Systems (1 mentions)
Triglyceride determination kit, by Merck Group (1 mentions)
Af1857 sp, by R&D Systems (1 mentions)
15 protocols using mouse albumin elisa quantification kit
1
Urinary Albumin and Glucose Quantification in Mice
2
Quantification of Urinary Albumin, Serum Creatinine, and Blood Urea Nitrogen
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Urinary albumin concentration was measured by using a mouse Albumin ELISA Quantification Kit (Bethyl Laboratories, Montgomery, TX). Serum creatinine was measured with the QuantiChrom Creatinine Assay Kit (cat.: DICT-500, Bioassay Systems, Hayward, CA), and blood urea nitrogen was measured with the QuantiChrom Urea Assay Kit (cat.: DIUR-500, Bioassay Systems) according to the manufacturer's instructions.
3
Urine Albumin and Kidney Assessment in Diabetic Mice
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Urine samples were collected by individually placing mice into metabolic cages (Iffa Credo, Lyon, France) for 24 h during week 10 of the study. The albumin concentration in the urine was measured by using a mouse albumin ELISA quantification kit (Bethyl Laboratories, Montgomery, TX, USA). Blood glucose, glycated haemoglobin, and systolic blood pressure were measured, as described previously [16 (link),20 (link)]. A mouse cystatin C ELISA kit (BioVendor, Brno, Czech Republic) was used to measure plasma cystatin C levels according to the manufacturer’s instructions. Mice were anesthetized via i.p. injection of sodium pentobarbital (100 mg/kg body weight; Euthatal; Sigma-Aldrich, Castle Hill, Australia) after 10 weeks (at 16 weeks of age). Both right and left kidneys were subsequently dissected, weighed and fixed in 10% formalin and embedded in paraffin as well as fresh frozen in liquid nitrogen for storage at −80 °C. Only diabetic mice with a blood glucose of ≥15 mmol/l were included in the experiments; mice with a blood glucose of <15 mmol/l and with polycystic kidneys were excluded from the study (<5% of the total number of mice).
4
Evaluating Diabetic Kidney Disease in Mice
5
Multiplex Cytokine and Chemokine Detection
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Levels of cytokines and chemokines in lung homogenates or serum were determined using mouse multiplex kits (Invitrogen and Millipore) read on a Bio-Plex Multiplex 200 Luminex reader (Bio-Rad). Levels of serum albumin in the BAL fluid were determined using a Mouse Albumin ELISA Quantification Kit as per manufacturer’s instructions (Bethyl Laboratories Inc.).
6
Quantification of Urinary Albumin and Creatinine in Mice
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Spot urine samples were collected from mice in the morning, immediately placed on ice, and subsequently frozen. Urinary albumin concentration was measured using the Mouse Albumin ELISA Quantification Kit (Bethyl Laboratories, Montgomery, TX) according to the manufacturer’s instructions. Urinary creatinine was measured using a creatinine assay kit from Cayman Chemical Company (Ann Arbor, MI). The kit uses a picric acid–based method. All samples were analyzed at least in duplicate. Albumin and creatinine concentrations were obtained using the standard curve equation of best fit, and the albumin/creatinine ratio was calculated in micrograms/milligram for each animal. For serum creatinine, mice were exsanguinated by cardiac puncture. Serum creatinine was measured using an enzymatic assay on the Olympus AU5822 chemistry system (Beckman Coulter) in the Biochemistry Laboratory at the McGill University Health Centre.
7
Quantifying Urinary Albumin and Creatinine
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Urinary albumin levels were measured using a mouse albumin ELISA quantification kit according to the manufacturer’s protocol (Bethyl Laboratories). Urinary creatinine levels were determined using a QuantiChrom Creatinine Assay kit according to the protocol (DICT-500, BioAssay System). Urinary proteins were also analyzed using SDS-PAGE after normalization to urinary creatinine.
8
Urine Albumin-to-Creatinine Ratio Assessment
9
Urinary Albumin and Creatinine Quantification
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Urinary albumin excretion was determined by Mouse Albumin ELISA quantification Kit (Bethyl laboratories, Inc., Montgomery, TX, USA) according to the manufactory's instruction.
Urine creatinine was measured by isocratic high-performance liquid chromatography (HPLC) on a Zorbax SCX300 column (Agilent, USA) using a slight modification of a method first reported by Yuen et al. [33 (link)]. In brief, 5 μL urine was added to 100 μL acetonitrile containing 0.5% acetic acid and vortexed for 15 seconds to extract the creatinine. After 15 min of −20°C storage and centrifugation the supernatants were evaporated and then reconstituted with 25 μL 5 mM sodium acetate, pH 4.1. The samples were centrifuged for 10 min at 3000 rpm. Duplicate samples (10 ul each) were fractionated on a 50 mm × 2.1 mm Zorbax SCX300 column with an in-front SCX guard column. Isocratic HPLC was performed at a flow rate of 1 mL/min, and UV absorbance was monitored at 225 nm. A standard curve was created by including a 2-fold dilution series of creatinine anhydrous (Sigma Aldrich). All aqueous solutions were filtered through a 0.22-micron filter before use.
Urine creatinine was measured by isocratic high-performance liquid chromatography (HPLC) on a Zorbax SCX300 column (Agilent, USA) using a slight modification of a method first reported by Yuen et al. [33 (link)]. In brief, 5 μL urine was added to 100 μL acetonitrile containing 0.5% acetic acid and vortexed for 15 seconds to extract the creatinine. After 15 min of −20°C storage and centrifugation the supernatants were evaporated and then reconstituted with 25 μL 5 mM sodium acetate, pH 4.1. The samples were centrifuged for 10 min at 3000 rpm. Duplicate samples (10 ul each) were fractionated on a 50 mm × 2.1 mm Zorbax SCX300 column with an in-front SCX guard column. Isocratic HPLC was performed at a flow rate of 1 mL/min, and UV absorbance was monitored at 225 nm. A standard curve was created by including a 2-fold dilution series of creatinine anhydrous (Sigma Aldrich). All aqueous solutions were filtered through a 0.22-micron filter before use.
10
Quantifying Lung Inflammation Biomarkers
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