The largest database of trusted experimental protocols

Inspexio smx 100ct

Manufactured by Shimadzu
Sourced in Japan

The InspeXio SMX-100CT is a computed tomography (CT) scanning system designed for laboratory use. It provides high-resolution 3D imaging capabilities for non-destructive analysis of various samples. The core function of the InspeXio SMX-100CT is to capture detailed X-ray images and generate 3D reconstructions of the internal structures of the analyzed specimens.

Automatically generated - may contain errors

29 protocols using inspexio smx 100ct

1

Micro-CT Analysis of Wrist Anatomy

Check if the same lab product or an alternative is used in the 5 most similar protocols
Thirteen wrists, including hands from nine Japanese cadavers, were obtained for this study. All cadavers were donated to the Department of Anatomy of the Tokyo Medical and Dental University. The study design was approved by the Ethics Committee at our institution.
We fixed all cadavers in 8% formalin and preserved them in 30% ethanol. Specimens with remarkable deformation around the distal radioulnar joint or remarkable instability of the distal radioulnar joint were excluded. We sectioned all specimens to obtain the wrist using a diamond band pathology saw (EXAKT 312; EXAKTAdvanced Technologies). We obtained three‐dimensional images of the 13 wrist blocks using a micro‐computed tomography (micro‐CT) scanner (inspeXio SMX‐100 CT; SHIMADZU) and application software (VGStudio Max 2.0). The micro‐CT parameters were as follows: voltage 100 kV, current 80 µA current, source‐to‐detector distance 700 mm, source‐to‐rotation center distance 500 mm, pitch 0.179, slice thickness 0.200 mm, field of view (xy) 91.55 mm, field of view (z) 45.0 mm, matrix 512 x 512, voxel size 0.179 mm/voxel. After obtaining three‐dimensional images, one specimen was found to have severe calcification on the distal ulna. Therefore, we used 12 specimens (three right and nine left) from nine cadavers (two males and seven females; average age, 83.6 years; age range, 49–96 years) for the analyses.
+ Open protocol
+ Expand
2

Micro-CT Analysis of Granular Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols
X-Ray CT of the samples was conducted using an inspeXio SMX-100CT (Shimadzu) equipped with a sealed tube type micro focus X-ray generator with a maximum output of 100 kV, and a high-sensitivity image intensifier. The sample granules were positioned between the X-ray generator and the X-ray detector, and X-ray fluoroscopic data was collected from every angle by rotating the sample through 360°. Finally, computed tomographic images (CT images) were calculated from the obtained data.
+ Open protocol
+ Expand
3

Micro-CT Analysis of Elbow Bone Morphology

Check if the same lab product or an alternative is used in the 5 most similar protocols
In 17 elbows for macroscopic observations, we randomly selected seven elbows for observation of the bone morphology. We took three‐dimensional (3D) images using a micro‐computed tomography (CT) scanner (inspeXio SMX‐100CT, Shimadzu, Kyoto, Japan) with application software (VGStudio Max 2.0, Heidelberg, Germany). To identify the relationship between the ST and the tendinous structures of the FPMs, radiopaque markers (1 mm2) were placed at 5 mm intervals along the corner of the anterior bases of the tendinous septa (TS), which were described below.
+ Open protocol
+ Expand
4

Micro-CT Imaging Protocol for Structural Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
A micro-computed tomography system (inspeXio SMX-100CT; Shimadzu Co., Kyoto, Japan) was used to capture the images at a voltage of 75 kV and current of 30 μA. The scanned data were reconstructed into two and three-dimensional images using an image analysis software (TRI/3D-BON; Ratoc System Engineering Co., Ltd., Tokyo, Japan).
+ Open protocol
+ Expand
5

3D Micro-CT Analysis of Ferret Maxillary Incisors

Check if the same lab product or an alternative is used in the 5 most similar protocols
We performed 3D micro-CT scans (inspeXio SMX-100CT; Shimadzu, Kyoto, Japan) on the maxillary incisors of ferrets, 13 weeks after birth. We converted CB files [512 × 512 pixels, 8 bits; voxel size, x:y:z = 1:1:1 (~0.06 mm per side)] to TIFF files, and 3D images were reconstructed and analyzed using computer imaging software (VGSTUDIO MAX; Volume Graphics GmbH., Heidelberg, Germany).
+ Open protocol
+ Expand
6

Micro-CT and 3D-VR Imaging of Transplanted Kidneys

Check if the same lab product or an alternative is used in the 5 most similar protocols
We performed 3D micro-CT scans (inspeXio SMX-100CT; Shimadzu, Kyoto, Japan) on the kidneys of mice at 19 days post-renal capsule transplantation. We converted CB files [512 × 512 pixels, 8 bits; voxel size, x:y:z = 1:1:1 (~ 0.06 mm/side)] to TIFF files, and 3D images were reconstructed and analyzed using computer imaging software (VGSTUDIO MAX 3.2; Volume Graphics GmbH., Heidelberg, Germany).
Additionally, 3D-VR image of H&E-stained sections were reconstructed and analyzed using computer imaging software (Avizo 2019.2; Thermo Fisher Scientific, Waltham, MA, USA).
+ Open protocol
+ Expand
7

In-vivo Imaging of Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols
For X-ray and μCT imaging, mice were anesthetized with a mixture of medetomidine, midazolam, and butorphanol. X-ray images were scanned using DX-50 (Faxitron Bioptics, Tuscon, AZ, USA). μCT images were scanned using an X-ray CT system (inspeXio SMX-100CT, Shimadzu, Kyoto) and analyzed with TRI/3D-BON software (Ratoc System Engineering Co., Ltd., Tokyo, Japan) according to the manufacturer’s instructions. Investigation was completed under a blinded condition of the experimental groups.
+ Open protocol
+ Expand
8

Comprehensive Tablet Characterization Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Each of the 200 mg tablets was compressed using a Handtab-100 (Ichihashi Seiki, Kyoto, Japan) for tablet, with 8 mm diameter flat-faced punches. The compressing force and dwell time in tablet compression were 60 MPa and 5 s, respectively. The hardness on a PC-30 tablet hardness scale (Okada Seiko, Tokyo, Japan) was recorded.
Each tablet was scanned by a microfocus X-ray CT system, inspeXio SMX-100CT (Shimadzu, Kyoto, Japan), under a 90 kV × 110 μA condition. The scanning view number was 600, and images was acquired by 3 scans at 16 μm resolution. The image size was 8.265 × 8.265 × 8.265 mm3 (512 × 512 × 512 voxels: xyz dimension). The high absorption area was mapped using the VGStudioMAX 3.0 software package (Volume Graphics, Heidelberg, Germany). The mapping range was 38,300–40,000 gray value.
Dissolution testing of the tablet was performed in 900 mL water medium (37.5 ± 0.5 °C) using a DT-610 apparatus and V-530 spectrometer (JASCO, Tokyo, Japan). The paddle speed was 50 rpm and non-sink conditions were employed. Aliquots at the desired timepoints were automatically removed and the THP concentration was instantly measured using a spectrometer at 285 nm. Furthermore, the disintegration time of each tablet was measured during the dissolution testing.
+ Open protocol
+ Expand
9

Quantifying Bone Mineral Loss by μCT

Check if the same lab product or an alternative is used in the 5 most similar protocols
Before and after demineralization, all specimens were scanned by µCT (inspeXio SMX-100CT; Shimadzu, Kyoto, Japan). The specimens were scanned with a spatial resolution of 8.1 µm and their projection images were collected at 40 kV and 100 µA using 180° rotation with 0.6° per projection step. An aluminum filter with thickness of 0.1 mm was placed in the beam path to remove low-energy radiation. Data were acquired with 512 × 512 pixel resolution and 8.1 µm isotropic voxel sizes. For calibrating mineral density, a series of reference phantoms were also scanned, which included four hydroxyapatite disks with different concentrations (100, 200, 300, 400 mg/cm3) and an aluminum pole (1550 mg/cm3).
Two-dimensional images of the specimens were reconstructed using a CT-analyzer software (CT-solver; Shimadzu, Kyoto, Japan). The stacked images were analyzed with Image J software (NIH, Bethesda, MD, USA) to produce an overall mineral profile of the two lines, which were the identical locations as scanned by the PIXE/PIGE system. Mineral loss (mg/cm3 · µm) was calculated by integrating the difference of the mineral content profiles of the identical specimens before and after demineralization.
+ Open protocol
+ Expand
10

Micro-CT Analysis of Ca(OH)2 Removal

Check if the same lab product or an alternative is used in the 5 most similar protocols
Micro-computed tomography (micro-CT; inspeXio SMX-100CT, Shimadzu, Tokyo, Japan) was performed before and after irrigation at 70 kV, 30 μA, voxel size 8.6 μm, 360° around the vertical axis, and 600 views. The volume of Ca(OH)2 (mm3) was determined using TRI/3D-BON software (Ratoc System Engineering, Tokyo, Japan), and the Ca(OH)2 removal rate was calculated as follows: Ca(OH)2 removal rate (%) = [1 - Ca(OH)2 volume after irrigation/Ca(OH)2 volume before irrigation] × 100.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!