Rottlerin

Manufactured by Cayman Chemical

Sourced in United States

Rottlerin is a chemical compound that can be used as a laboratory reagent. It functions as a selective inhibitor of the protein kinase C delta (PKCδ) enzyme.

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Lab products found in correlation

3 protocols using rottlerin

Integrin-mediated Signaling Pathway Analysis

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All reagents unless otherwise specified were from Sigma-Aldrich. Wortmannin, Ly294002, rottlerin, bisindolylmaleimide i, bisindolylmaleimide ix, Gö6983 were from Cayman Chemical. P11, GRGDSP, GRADSP, αvβ3 function blocking antibody (LM609) were from EMD Millipore. PP1, PP2, PP3 were from Calbiochem. LDH assay was from Promega. Phospho-MARCKS Ser152/156 (Cat#2741), MARCKS (Cat#5607), Phospho-PI3K (Cat#4228), p85 PI3K (Cat#4257), Phospho-PKCδ (Cat#2055), PKCd (Cat#9616) antibodies were from Cell Signaling Technology. FITC-PIP3 antibody (Cat#G345) was from Echelon. All secondary antibodies and isotype controls were from Life Technologies.

Cornick S., Moreau F, & Chadee K. (2016). Entamoeba histolytica Cysteine Proteinase 5 Evokes Mucin Exocytosis from Colonic Goblet Cells via αvβ3 Integrin. PLoS Pathogens, 12(4), e1005579.

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Neutrophil Inhibitor Evaluation for Candida

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Neutrophils were pre-treated with indicated inhibitors 30 min before addition of C. albicans. Inhibitors SkyI (for Syk), BAPTA-AM (a selective chelator of Ca²⁺), Ro 318220 (for total PKC), Rottlerin (for PKCδ), BB-CI-Amidine (for PAD1-4), GSK484 (for PAD4) were all purchased from Cayman. Ro 6976 (for PKCα+β1), LY 333531 (for PKCβ) were from Millipore.

Wu S.Y., Weng C.L., Jheng M.J., Kan H.W., Hsieh S.T., Liu F.T, & Wu-Hsieh B.A. (2019). Candida albicans triggers NADPH oxidase-independent neutrophil extracellular traps through dectin-2. PLoS Pathogens, 15(11), e1008096.

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Intestinal Barrier Regulation by PKC Inhibitors

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Caco-2 cells seeded on Millicells and mounted in Ussing chambers were treated apically with inhibitors for 30-60 min prior to the apical challenge of RGal (1000 μg ml À1 ). The pan PKC inhibitor, GF109203X (500 nM, Tocris), the myristoylated PKCζ pseudosubstrate inhibitor (10 μM, Enzo Life Sciences, Farmingdale, NY, USA), the PKCα/β inhibitor, Go6976 (10 nM, Tocris), the PKCδ inhibitor, Rottlerin (3 μM, Cayman Chemical), the MEK1/2 inhibitor, U0126 (20 μM, Cayman Chemical), and the p38 inhibitor SB203580 (20 μM, Cayman Chemical) were added 30 min before RGal addition. The TLR4 inhibitor C34 (100 μg ml À1 ) was added apically 1 h before mounting in Ussing chambers.

Baggio C.H., Shang J., Gordon M.H., Stephens M., von der Weid P.Y., Nascimento A.M., Román Y., Cipriani T.R, & MacNaughton W.K. (2022). The dietary fibre rhamnogalacturonan improves intestinal epithelial barrier function in a microbiota-independent manner. British journal of pharmacology, 179(2).

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